| http://purl.uniprot.org/citations/12427989 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12427989 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12427989 | http://www.w3.org/2000/01/rdf-schema#comment | "GSK3 is a highly conserved kinase that negatively regulates many cellular processes by phosphorylating a variety of protein substrates. BIN2 is a GSK3-like kinase in Arabidopsis that functions as a negative regulator of brassinosteroid (BR) signaling. It was proposed that BR signals, perceived by a membrane BR receptor complex that contains the leucine (Leu)-rich repeat receptor-like kinase BRI1, inactivate BIN2 to relieve its inhibitory effect on unknown downstream BR-signaling components. Using a yeast (Saccharomyces cerevisiae) two-hybrid approach, we discovered a potential BIN2 substrate that is identical to a recently identified BR-signaling protein, BES1. BES1 and its closest homolog, BZR1, which was also uncovered as a potential BR-signaling protein, display specific interactions with BIN2 in yeast. Both BES1 and BZR1 contain many copies of a conserved GSK3 phosphorylation site and can be phosphorylated by BIN2 in vitro via a novel GSK3 phosphorylation mechanism that is independent of a priming phosphorylation or a scaffold protein. Five independent bes1 alleles containing the same proline-233-Leu mutation were identified as semidominant suppressors of two different bri1 mutations. Over-expression of the wild-type BZR1 gene partially complemented bin2/+ mutants and resulted in a BRI1 overexpression phenotype in a BIN2(+) background, whereas overexpression of a mutated BZR1 gene containing the corresponding proline-234-Leu mutation rescued a weak bri1 mutation and led to a bes1-like phenotype. Confocal microscopic analysis indicated that both BES1 and BZR1 proteins were mainly localized in the nucleus. We propose that BES1/BZR1 are two nuclear components of BR signaling that are negatively regulated by BIN2 through a phosphorylation-initiated process."xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.org/dc/terms/identifier | "doi:10.1104/pp.102.010918"xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.org/dc/terms/identifier | "doi:10.1104/pp.102.010918"xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/author | "Li J."xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/author | "Li J."xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/author | "Zhao J."xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/author | "Zhao J."xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/author | "Schmitz R.J."xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/author | "Schmitz R.J."xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/author | "Peng P."xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/author | "Peng P."xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/author | "Tax F.E."xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/author | "Tax F.E."xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/author | "Decker A.D."xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/author | "Decker A.D."xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/name | "Plant Physiol."xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/name | "Plant Physiol."xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/pages | "1221-1229"xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/pages | "1221-1229"xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/title | "Two putative BIN2 substrates are nuclear components of brassinosteroid signaling."xsd:string |
| http://purl.uniprot.org/citations/12427989 | http://purl.uniprot.org/core/title | "Two putative BIN2 substrates are nuclear components of brassinosteroid signaling."xsd:string |