| http://purl.uniprot.org/citations/12810715 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12810715 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12810715 | http://www.w3.org/2000/01/rdf-schema#comment | "Apolipoprotein A-V (apoA-V), the newest member of the plasma apolipoprotein family, was recently discovered by comparison of the mouse and human genomes. Studies in rodents and population surveys of human apoA-V polymorphisms have noted a strong effect of apoA-V on plasma triglyceride levels. Toward the elucidation of the biologic function of apoA-V, we used spectroscopic and surface chemistry techniques to probe its structure and interfacial activity. Computer-assisted sequence analysis of apoA-V predicts that it is very hydrophobic, contains a significant amount of alpha-helical secondary structure, and probably is composed of discrete structural regions with varying degrees of lipid affinity. Fluorescence spectroscopy of recombinant human apoA-V provided evidence of tertiary folding, and light scattering studies indicated that apoA-V transforms dimyristoylphosphatidylcholine vesicles into discoidal complexes with an efficiency similar to that of apoA-I. Surface chemistry techniques revealed that apoA-V displays high affinity, low elasticity, and slow binding kinetics at hydrophobic interfaces, properties we propose may retard triglyceride-rich particle assembly. Metabolic labeling and immunofluorescence studies of COS-1 cells transfected with human apoA-V demonstrated that apoA-V is poorly secreted, remains associated with the endoplasmic reticulum, and does not traffic to the Golgi. Given that overexpression of the apoA-V gene lowers plasma triglycerides in mice, these data together suggest that apoA-V may function intracellularly to modulate hepatic VLDL synthesis and/or secretion."xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m303784200"xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m303784200"xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/author | "Ryan R.O."xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/author | "Ryan R.O."xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/author | "Beckstead J.A."xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/author | "Beckstead J.A."xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/author | "Cook V.R."xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/author | "Cook V.R."xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/author | "Gallagher J.W."xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/author | "Gallagher J.W."xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/author | "Martin D.D.O."xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/author | "Martin D.D.O."xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/author | "Shelness G.S."xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/author | "Shelness G.S."xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/author | "Weinberg R.B."xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/author | "Weinberg R.B."xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/pages | "34438-34444"xsd:string |
| http://purl.uniprot.org/citations/12810715 | http://purl.uniprot.org/core/pages | "34438-34444"xsd:string |