| http://purl.uniprot.org/citations/12818200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12818200 | http://www.w3.org/2000/01/rdf-schema#comment | "Bloom syndrome protein forms an oligomeric ring structure and belongs to a group of DNA helicases showing extensive homology to the Escherichia coli DNA helicase RecQ, a suppressor of illegitimate recombination. After over-production in E.coli, we have purified the RecQ core of BLM consisting of the DEAH, RecQ-Ct and HRDC domains (amino acid residues 642-1290). The BLM(642-1290) fragment could function as a DNA-stimulated ATPase and as a DNA helicase, displaying the same substrate specificity as the full-size protein. Gel-filtration experiments revealed that BLM(642-1290) exists as a monomer both in solution and in its single-stranded DNA-bound form, even in the presence of Mg(2+) and ATPgammaS. Rates of ATP hydrolysis and DNA unwinding by BLM(642-1290) showed a hyperbolic dependence on ATP concentration, excluding a co-operative interaction between ATP-binding sites. Using a lambda Spi(-) assay, we have found that the BLM(642-1290) fragment is able to partially substitute for the RecQ helicase in suppressing illegitimate recombination in E.coli. A deletion of 182 C-terminal amino acid residues of BLM(642-1290), including the HRDC domain, resulted in helicase and single-stranded DNA-binding defects, whereas kinetic parameters for ATP hydrolysis of this mutant were close to the BLM(642-1290) values. This confirms the prediction that the HRDC domain serves as an auxiliary DNA-binding domain. Mutations at several conserved residues within the RecQ-Ct domain of BLM reduced ATPase and helicase activities severely as well as single-stranded DNA-binding of the enzyme. Together, these data define a minimal helicase domain of BLM and demonstrate its ability to act as a suppressor of illegitimate recombination."xsd:string |
| http://purl.uniprot.org/citations/12818200 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0022-2836(03)00534-5"xsd:string |
| http://purl.uniprot.org/citations/12818200 | http://purl.uniprot.org/core/author | "Bickle T.A."xsd:string |
| http://purl.uniprot.org/citations/12818200 | http://purl.uniprot.org/core/author | "Ikeda H."xsd:string |
| http://purl.uniprot.org/citations/12818200 | http://purl.uniprot.org/core/author | "Imai Y."xsd:string |
| http://purl.uniprot.org/citations/12818200 | http://purl.uniprot.org/core/author | "Hamburger F."xsd:string |
| http://purl.uniprot.org/citations/12818200 | http://purl.uniprot.org/core/author | "Janscak P."xsd:string |
| http://purl.uniprot.org/citations/12818200 | http://purl.uniprot.org/core/author | "Shiraishi K."xsd:string |
| http://purl.uniprot.org/citations/12818200 | http://purl.uniprot.org/core/author | "Garcia P.L."xsd:string |
| http://purl.uniprot.org/citations/12818200 | http://purl.uniprot.org/core/author | "Makuta Y."xsd:string |
| http://purl.uniprot.org/citations/12818200 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12818200 | http://purl.uniprot.org/core/name | "J Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/12818200 | http://purl.uniprot.org/core/pages | "29-42"xsd:string |
| http://purl.uniprot.org/citations/12818200 | http://purl.uniprot.org/core/title | "Characterization and mutational analysis of the RecQ core of the bloom syndrome protein."xsd:string |
| http://purl.uniprot.org/citations/12818200 | http://purl.uniprot.org/core/volume | "330"xsd:string |
| http://purl.uniprot.org/citations/12818200 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12818200 |
| http://purl.uniprot.org/citations/12818200 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12818200 |
| http://purl.uniprot.org/uniprot/P54132#attribution-D0047410E948FC377118601EF51D3CFB | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/12818200 |
| http://purl.uniprot.org/uniprot/#_P54132-mappedCitation-12818200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12818200 |
| http://purl.uniprot.org/uniprot/#_B2RAN0-mappedCitation-12818200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12818200 |
| http://purl.uniprot.org/uniprot/#_B7Z8C1-mappedCitation-12818200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12818200 |
| http://purl.uniprot.org/uniprot/#_B7ZKN7-mappedCitation-12818200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12818200 |
| http://purl.uniprot.org/uniprot/#_B7Z2X2-mappedCitation-12818200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12818200 |
| http://purl.uniprot.org/uniprot/#_Q3B7X0-mappedCitation-12818200 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12818200 |