| http://purl.uniprot.org/citations/1283219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1283219 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1283219 | http://www.w3.org/2000/01/rdf-schema#comment | "The cloned human delayed rectifying K+ channel Kv2.1 (drk1) was expressed in clonal mouse fibroblasts (L-cells) and rat basophilic leukemia cells (RBL-1) by direct cytoplasmic microinjection of complementary RNA (cRNA). Within six hours, cells microinjected with Kv2.1 cRNA expressed a large sustained outward current as determined from whole-cell patch-clamp recordings. Nearly 100% of cells injected with cRNA expressed outward current. Current density was 30-70 pA/pF when measured at a potential of +50 mV. Steady-state activation and inactivation parameters for Kv2.1 were similar when expressed in either L-cells or RBL-1 cells. These results are the first to demonstrate that functional ion channel proteins can be expressed in mammalian clonal cell lines by direct cytoplasmic microinjection of cRNA."xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.org/dc/terms/identifier | "doi:10.1007/bf00370422"xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.org/dc/terms/identifier | "doi:10.1007/bf00370422"xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/author | "Lewis D.L."xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/author | "Lewis D.L."xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/author | "Ikeda S.R."xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/author | "Ikeda S.R."xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/author | "Joho R.H."xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/author | "Joho R.H."xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/author | "Soler F."xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/author | "Soler F."xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/author | "Zuhlke R.D."xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/author | "Zuhlke R.D."xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/name | "Pflugers Arch."xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/name | "Pflugers Arch."xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/pages | "201-203"xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/pages | "201-203"xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/title | "Heterologous expression of the human potassium channel Kv2.1 in clonal mammalian cells by direct cytoplasmic microinjection of cRNA."xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/title | "Heterologous expression of the human potassium channel Kv2.1 in clonal mammalian cells by direct cytoplasmic microinjection of cRNA."xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/volume | "422"xsd:string |
| http://purl.uniprot.org/citations/1283219 | http://purl.uniprot.org/core/volume | "422"xsd:string |