| http://purl.uniprot.org/citations/12890687 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12890687 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12890687 | http://www.w3.org/2000/01/rdf-schema#comment | "Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes/vacuoles. The formation of autophagosomes involves a dynamic rearrangement of the membrane for which two ubiquitin-like modifications (the conjugation of Apg12p and the modification of a soluble form of MAP-LC3 to a membrane-bound form) are essential. In yeast, Apg10p is an E2-like enzyme essential for Apg12p conjugation. The isolated mouse APG10 gene product interacts with mammalian Apg12p dependent on mammalian Apg7p (E1-like enzyme), and facilitates Apg12p conjugation. The interaction of Apg10p with Apg12p is dependent on the carboxyl-terminal glycine of Apg12p. Mutational analysis of the predicted active site cysteine (Cys161) within mouse Apg10p shows that mutant Apg10pC161S, which can form a stable intermediate with Apg12p, inhibits Apg12p conjugation even in the presence of Apg7p, while overexpression of Apg7p facilitates formation of an Apg12p-Apg5p conjugate. Furthermore, the coexpression of Apg10p with Apg7p facilitates the modification of a soluble form of MAP-LC3 to a membrane-bound form, a second modification essential for autophagy. Mouse Apg10p interacts with MAP-LC3 in HEK293 cells, while no mutant Apg10pC161S forms any intermediate with MAP-LC3. Direct interaction between Apg10p and MAP-LC3 is also demonstrated by yeast two-hybrid analysis. The inability of mutant Apg10pC161S to form any intermediate with MAP-LC3 has ruled out the possibility that MAP-LC3 interacts with Apg10p as a substrate."xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m300550200"xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m300550200"xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/author | "Ueno T."xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/author | "Ueno T."xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/author | "Nemoto T."xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/author | "Nemoto T."xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/author | "Yokota M."xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/author | "Yokota M."xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/author | "Ohsumi M."xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/author | "Ohsumi M."xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/author | "Kominami E."xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/author | "Kominami E."xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/author | "Tanida I."xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/author | "Tanida I."xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/author | "Minematsu-Ikeguchi N."xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/author | "Minematsu-Ikeguchi N."xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/author | "Tanida-Miyake E."xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/author | "Tanida-Miyake E."xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12890687 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |