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| http://purl.uniprot.org/citations/12911595 | http://www.w3.org/2000/01/rdf-schema#comment | "We previously localized the heparin binding region on high molecular weight kininogen to domain 5 (D5) by quantifying the binding using surface plasmon resonance of D5 fused at its N-terminal to glutathione-S-transferase. We further examined GST-(H475-S626) which at 100 nm was previously shown to be ineffective in reversing the heparin acceleration of antithrombin inhibition of thrombin. However, we now show that at a concentration of 400 nm, complete reversal of accelerated inhibition occurred. To characterize the interacting sequences on D5, four peptides representing surface loops of a molecular model were synthesized. Peptides H475-H485 and G440-G455, rich in histidine and low in lysine, showed weak or no detectable binding in the absence of Zn++, but tighter binding in the presence of Zn++. H483-K497 containing three histidines and six lysines showed tight binding without Zn++, and increased in avidity with Zn++. In contrast, G486-K502, low in histidine and high in lysine, showed tight binding (KD = 0.8 microm) in the absence and presence of Zn++. Both H483-K497 and G486-K502 were effective in neutralizing the accelerated inhibition by heparin of thrombin by antithrombin in the absence of Zn++. Therefore, a set of lysine residues in the sequence of K487-K502 is responsible for Zn++-independent binding of heparin. Further, a group of histidine residues in sequence range of H475-H485 contributes to Zn++-dependent binding of heparin to HK-D5."xsd:string |
| http://purl.uniprot.org/citations/12911595 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1538-7836.2003.00291.x"xsd:string |
| http://purl.uniprot.org/citations/12911595 | http://purl.uniprot.org/core/author | "Lin Y."xsd:string |
| http://purl.uniprot.org/citations/12911595 | http://purl.uniprot.org/core/author | "Colman R.W."xsd:string |
| http://purl.uniprot.org/citations/12911595 | http://purl.uniprot.org/core/author | "Pixley R.A."xsd:string |
| http://purl.uniprot.org/citations/12911595 | http://purl.uniprot.org/core/author | "Isordia-Salas I."xsd:string |
| http://purl.uniprot.org/citations/12911595 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12911595 | http://purl.uniprot.org/core/name | "J Thromb Haemost"xsd:string |
| http://purl.uniprot.org/citations/12911595 | http://purl.uniprot.org/core/pages | "1791-1798"xsd:string |
| http://purl.uniprot.org/citations/12911595 | http://purl.uniprot.org/core/title | "Fine mapping of the sequences in domain 5 of high molecular weight kininogen (HK) interacting with heparin and zinc."xsd:string |
| http://purl.uniprot.org/citations/12911595 | http://purl.uniprot.org/core/volume | "1"xsd:string |
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