| http://purl.uniprot.org/citations/12954649 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12954649 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12954649 | http://www.w3.org/2000/01/rdf-schema#comment | "The Shank/proline-rich synapse-associated protein family of multidomain proteins is known to play an important role in the organization of synaptic multiprotein complexes. For instance, the Shank PDZ domain binds to the C termini of guanylate kinase-associated proteins, which in turn interact with the guanylate kinase domain of postsynaptic density-95 scaffolding proteins. Here we describe the crystal structures of Shank1 PDZ in its peptide free form and in complex with the C-terminal hexapeptide (EAQTRL) of guanylate kinase-associated protein (GKAP1a) determined at 1.8- and 2.25-A resolutions, respectively. The structure shows the typical class I PDZ interaction of PDZ-peptide complex with the consensus sequence -X-(Thr/Ser)-X-Leu. In addition, Asp-634 within the Shank1 PDZ domain recognizes the positively charged Arg at -1 position and hydrogen bonds, and salt bridges between Arg-607 and the side chains of the ligand at -3 and -5 positions contribute further to the recognition of the peptide ligand. Remarkably, whether free or complexed, Shank1 PDZ domains form dimers with a conserved beta B/beta C loop and N-terminal beta A strands, suggesting a novel model of PDZ-PDZ homodimerization. This implies that antiparallel dimerization through the N-terminal beta A strands could be a common configuration among PDZ dimers. Within the dimeric structure, the two-peptide binding sites are arranged so that the N termini of the bound peptide ligands are in close proximity and oriented toward the 2-fold axis of the dimer. This configuration may provide a means of facilitating dimeric organization of PDZ-target assemblies."xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m306919200"xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m306919200"xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/author | "Kim E."xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/author | "Kim E."xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/author | "Lee J.H."xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/author | "Lee J.H."xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/author | "Park S.H."xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/author | "Park S.H."xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/author | "Park S.J."xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/author | "Park S.J."xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/author | "Eom S.H."xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/author | "Eom S.H."xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/author | "Kang G.B."xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/author | "Kang G.B."xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/author | "Im Y.J."xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/author | "Im Y.J."xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/author | "Rho S.-H."xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/author | "Rho S.-H."xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12954649 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |