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http://purl.uniprot.org/citations/1335101http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/1335101http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/1335101http://www.w3.org/2000/01/rdf-schema#comment"Using the polymerase chain reaction (PCR), we identified RNA transcripts for two distinct classes of neuronal-type voltage-gated Ca2+ channels (VGCC) in a prototypic small cell lung carcinoma (SCLC) cell line, SCC-9. Oligonucleotide primers were designed to encode amino acid sequences common to alpha 1-subunits of known neuronal VGCC classes. Sequencing of complementary DNA (cDNA) clones derived from two independent PCR products revealed that one corresponded to a brain class A VGCC fragment predicted to encode a P-type VGCC (insensitive to dihydropyridines and omega-conotoxin) characteristic of cerebellar Purkinje cells but not previously identified in humans. The second PCR product was identical (except for one conservative nucleotide difference) to a fragment of the class D VGCC of neurons and neuroendocrine cells, which encodes an L-type VGCC (sensitive to dihydropyridines). By Northern blot analyses, both cDNAs hybridized to messenger RNAs (mRNAs) obtained from SCC-9; class D hybridized additionally to human cerebral cortical mRNA, but neither hybridized to mRNA from the skeletal muscle cell line TE671. Although no cDNA corresponding to class B VGCC (N-type) was identified, SCLCs are known to express VGCC that are sensitive to omega-conotoxin and coprecipitate with 125I-labeled-omega-conotoxin when complexed with serum IgG from patients with the Lambert-Eaton myasthenic syndrome. The multiple classes of neuronal-type VGCC expressed in SCLC could conceivably have both unique and related antigenic determinants that may give rise to antineuronal autoimmune responses. This would account for a spectrum of paraneoplastic neurologic disorders including the Lambert-Eaton syndrome and subacute cerebellar degeneration."xsd:string
http://purl.uniprot.org/citations/1335101http://purl.org/dc/terms/identifier"doi:10.1016/s0025-6196(12)61144-6"xsd:string
http://purl.uniprot.org/citations/1335101http://purl.org/dc/terms/identifier"doi:10.1016/s0025-6196(12)61144-6"xsd:string
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/author"Snutch T.P."xsd:string
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/author"Snutch T.P."xsd:string
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/author"Wieben E.D."xsd:string
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/author"Wieben E.D."xsd:string
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/author"Lennon V.A."xsd:string
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/author"Lennon V.A."xsd:string
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/author"Griesmann G.E."xsd:string
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/author"Griesmann G.E."xsd:string
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/author"Oguro-Okano M."xsd:string
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/author"Oguro-Okano M."xsd:string
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/author"Slaymaker S.J."xsd:string
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/author"Slaymaker S.J."xsd:string
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/date"1992"xsd:gYear
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/date"1992"xsd:gYear
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/name"Mayo Clin. Proc."xsd:string
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/name"Mayo Clin. Proc."xsd:string
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/pages"1150-1159"xsd:string
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/pages"1150-1159"xsd:string
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/title"Molecular diversity of neuronal-type calcium channels identified in small cell lung carcinoma."xsd:string
http://purl.uniprot.org/citations/1335101http://purl.uniprot.org/core/title"Molecular diversity of neuronal-type calcium channels identified in small cell lung carcinoma."xsd:string
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