| http://purl.uniprot.org/citations/14568990 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14568990 | http://www.w3.org/2000/01/rdf-schema#comment | "A thiol-reactive membrane-associated protein (TRAP) binds covalently to the cytoplasmic domain of the human insulin receptor (IR) beta-subunit when cells are treated with the homobifunctional cross-linker reagent 1,6-bismaleimidohexane. Here, TRAP was found to be phospholipase C gamma1 (PLCgamma1) by mass spectrometry analysis. PLCgamma1 associated with the IR both in cultured cell lines and in a primary culture of rat hepatocytes. Insulin increased PLCgamma1 tyrosine phosphorylation at Tyr-783 and its colocalization with the IR in punctated structures enriched in cortical actin at the dorsal plasma membrane. This association was found to be independent of PLCgamma1 Src homology 2 domains, and instead required the pleckstrin homology (PH)-EF-hand domain. Expression of the PH-EF construct blocked endogenous PLCgamma1 binding to the IR and inhibited insulin-dependent phosphorylation of mitogen-activated protein kinase (MAPK), but not AKT. Silencing PLCgamma1 expression using small interfering RNA markedly reduced insulin-dependent MAPK regulation in HepG2 cells. Conversely, reconstitution of PLCgamma1 in PLCgamma1-/-fibroblasts improved MAPK activation by insulin. Our results show that PLCgamma1 is a thiol-reactive protein whose association with the IR could contribute to the activation of MAPK signaling by insulin."xsd:string |
| http://purl.uniprot.org/citations/14568990 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.200301131"xsd:string |
| http://purl.uniprot.org/citations/14568990 | http://purl.uniprot.org/core/author | "Bernier M."xsd:string |
| http://purl.uniprot.org/citations/14568990 | http://purl.uniprot.org/core/author | "Jang H.J."xsd:string |
| http://purl.uniprot.org/citations/14568990 | http://purl.uniprot.org/core/author | "Kwon Y.K."xsd:string |
| http://purl.uniprot.org/citations/14568990 | http://purl.uniprot.org/core/author | "He H.J."xsd:string |
| http://purl.uniprot.org/citations/14568990 | http://purl.uniprot.org/core/author | "Kole S."xsd:string |
| http://purl.uniprot.org/citations/14568990 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/14568990 | http://purl.uniprot.org/core/name | "J Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/14568990 | http://purl.uniprot.org/core/pages | "375-384"xsd:string |
| http://purl.uniprot.org/citations/14568990 | http://purl.uniprot.org/core/title | "Role of the pleckstrin homology domain of PLCgamma1 in its interaction with the insulin receptor."xsd:string |
| http://purl.uniprot.org/citations/14568990 | http://purl.uniprot.org/core/volume | "163"xsd:string |
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