| http://purl.uniprot.org/citations/14652006 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14652006 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14652006 | http://www.w3.org/2000/01/rdf-schema#comment | "B and T lymphocytes express receptors providing positive and negative co-stimulatory signals. We recently identified a novel co-stimulatory molecule, B and T lymphocyte attenuator (BTLA), which exerts inhibitory effects on B and T lymphocytes. The cytoplasmic domain of murine and human BTLA share three conserved tyrosine-based signaling motifs, a Grb-2 recognition consensus, and two immunoreceptor tyrosine-based inhibitory motifs (ITIMs). Phosphorylation of the cytoplasmic domain of BTLA induced the association with the protein tyrosine phosphatases SHP-1 and SHP-2. Association of SHP-1 and SHP-2 to other receptors can involve recruitment to either a single receptor ITIM or to two receptor ITIMs. Here, we analyzed the requirements of BTLA interaction with SHP-1 and SHP-2 in a series of murine and human BTLA mutants. For human BTLA, mutations of either Y257 or Y282, but not Y226, abrogated association with both SHP-1 and SHP-2. For murine BTLA, mutation of either Y274 or Y299, but not Y245, also abrogated association with both SHP-1 and SHP-2. These results indicate that for both murine and human BTLA, association with SHP-1 or SHP-2 requires both of conserved ITIM motifs and does not involve the conserved Grb-2 consensus. Thus, similar to the bisphosphoryl tyrosine-based activation motif (BTAM) by which the Grb-2 associated binder (Gab1), PDGF receptor, and PECAM-1 recruit SHP-2, BTLA also relies on dual ITIMs for its association with the phosphatases SHP-1 and SHP-2."xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2003.11.070"xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2003.11.070"xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/author | "Watanabe N."xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/author | "Watanabe N."xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/author | "Murphy K.M."xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/author | "Murphy K.M."xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/author | "Murphy T.L."xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/author | "Murphy T.L."xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/author | "Gavrieli M."xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/author | "Gavrieli M."xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/author | "Loftin S.K."xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/author | "Loftin S.K."xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/pages | "1236-1243"xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/pages | "1236-1243"xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/title | "Characterization of phosphotyrosine binding motifs in the cytoplasmic domain of B and T lymphocyte attenuator required for association with protein tyrosine phosphatases SHP-1 and SHP-2."xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/title | "Characterization of phosphotyrosine binding motifs in the cytoplasmic domain of B and T lymphocyte attenuator required for association with protein tyrosine phosphatases SHP-1 and SHP-2."xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/volume | "312"xsd:string |
| http://purl.uniprot.org/citations/14652006 | http://purl.uniprot.org/core/volume | "312"xsd:string |