| http://purl.uniprot.org/citations/15001553 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15001553 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15001553 | http://www.w3.org/2000/01/rdf-schema#comment | "Vascular endothelial growth factor (VEGF) and its receptors are key regulators of angiogenesis and are potential targets in cancer therapy. Here we report the down-regulation of activated VEGF receptor (VEGFR)-1/Flt-1 by endocytosis and proteolytic degradation. VEGF stimulation induced a ternary complex of Flt-1, c-Cbl, and CD2AP. Substitution of tyrosine 1333 in Flt-1 with phenylalanine (Y1333F) impaired its binding to c-Cbl. In a transient expression system, VEGF stimulated colocalization of Flt-1, CD2AP, and c-Cbl in endocytic vesicles. This colocalization was significantly impaired by an inhibitor of VEGFR kinase SU5416, the Y1333F mutation in Flt-1, or by a dominant negative form of CD2AP. In Flt-1-overexpressing NIH3T3 cells, expression of the wild-type CD2AP enhanced VEGF-stimulated internalization as well as ubiquitination of Flt-1 whereas that of a mutated form of either CD2AP or c-Cbl failed to do so. These results suggest that the c-Cbl/CD2AP complex binds to activated Flt-1 and plays a crucial role in its endocytosis and subsequent degradation."xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.org/dc/terms/identifier | "doi:10.1096/fj.03-0767fje"xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.org/dc/terms/identifier | "doi:10.1096/fj.03-0767fje"xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/author | "Kobayashi S."xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/author | "Kobayashi S."xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/author | "Shibuya M."xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/author | "Shibuya M."xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/author | "Nojima Y."xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/author | "Nojima Y."xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/author | "Sawano A."xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/author | "Sawano A."xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/author | "Maru Y."xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/author | "Maru Y."xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/name | "FASEB J."xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/name | "FASEB J."xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/pages | "929-931"xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/pages | "929-931"xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/title | "The c-Cbl/CD2AP complex regulates VEGF-induced endocytosis and degradation of Flt-1 (VEGFR-1)."xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/title | "The c-Cbl/CD2AP complex regulates VEGF-induced endocytosis and degradation of Flt-1 (VEGFR-1)."xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/volume | "18"xsd:string |
| http://purl.uniprot.org/citations/15001553 | http://purl.uniprot.org/core/volume | "18"xsd:string |