| http://purl.uniprot.org/citations/15136565 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15136565 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15136565 | http://www.w3.org/2000/01/rdf-schema#comment | "Lipid droplets (LDs) are a class of ubiquitous cellular organelles that are involved in lipid storage and metabolism. Although the mechanisms of the biogenesis of LDs are still unclear, a set of proteins called the PAT domain family have been characterized as factors associating with LDs. Perilipin, a member of this family, is expressed exclusively in the adipose tissue and regulates the breakdown of triacylglycerol in LDs via its phosphorylation. In this study, we used a yeast two-hybrid system to examine the potential function of perilipin. We found direct interaction between perilipin and CGI-58, a deficiency of which correlated with the pathogenesis of Chanarin-Dorfman syndrome (CDS). Endogenous CGI-58 was distributed predominantly on the surface of LDs in differentiated 3T3-L1 cells, and its expression increased during adipocyte differentiation. Overexpressed CGI-58 tagged with GFP gathered at the surface of LDs and colocalized with perilipin. This interaction seems physiologically important because CGI-58 mutants carrying an amino acid substitution identical to that found in CDS lost the ability to be recruited to LDs. These mutations significantly weakened the binding of CGI-58 with perilipin, indicating that the loss of this interaction is involved in the etiology of CDS. Furthermore, we identified CGI-58 as a binding partner of ADRP, another PAT domain protein expressed ubiquitously, by yeast two-hybrid assay. GFP-CGI-58 expressed in non-differentiated 3T3-L1 or CHO-K1 cells was colocalized with ADRP, and the CGI-58 mutants were not recruited to LDs carrying ADRP, indicating that CGI-58 may also cooperate with ADRP."xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m403920200"xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m403920200"xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://purl.uniprot.org/core/author | "Matsushita S."xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://purl.uniprot.org/core/author | "Matsushita S."xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://purl.uniprot.org/core/author | "Yamaguchi T."xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://purl.uniprot.org/core/author | "Yamaguchi T."xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://purl.uniprot.org/core/author | "Osumi T."xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://purl.uniprot.org/core/author | "Osumi T."xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://purl.uniprot.org/core/author | "Omatsu N."xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://purl.uniprot.org/core/author | "Omatsu N."xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15136565 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15136565 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://purl.uniprot.org/core/pages | "30490-30497"xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://purl.uniprot.org/core/pages | "30490-30497"xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://purl.uniprot.org/core/title | "CGI-58 interacts with perilipin and is localized to lipid droplets. Possible involvement of CGI-58 mislocalization in Chanarin-Dorfman syndrome."xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://purl.uniprot.org/core/title | "CGI-58 interacts with perilipin and is localized to lipid droplets. Possible involvement of CGI-58 mislocalization in Chanarin-Dorfman syndrome."xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/15136565 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15136565 |
| http://purl.uniprot.org/citations/15136565 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15136565 |