| http://purl.uniprot.org/citations/15158712 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15158712 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15158712 | http://www.w3.org/2000/01/rdf-schema#comment | "Here, we demonstrate the presence of multiple isoforms of palate lung nasal epithelial clone (PLUNC) in human nasal lavage fluid (NLF). Eight isoforms were separated by two-dimensional gel electrophoresis (2-DE), and peptide mapping of the proteins was performed using MALDI-TOF MS (matrix assisted laser desorption/ionization time of flight mass spectrometry) of tryptic and asparginase cleavages. The identification was verified by amino acid sequencing after analysis of collision-induced dissociation (CID) fragmentation spectra with nanoelectrospray MS/MS. One isoform showed an electrophoretic mobility shift after N-glycosidase treatment, indicating that at least one of the PLUNC isoforms is glycosylated. We also demonstrate that PLUNC in NLF binds to lipopolysaccharide (LPS) in vitro; indeed, out of all proteins present in NLF only the PLUNC isoforms were found to adsorb to an LPS-coated surface. These results show that PLUNC is expressed as multiple LPS-binding isoforms in human NLF. The possibility that PLUNC may play a role in the innate immune response of the upper airways is inferred."xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbapap.2004.01.001"xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbapap.2004.01.001"xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://purl.uniprot.org/core/author | "Lindahl M."xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://purl.uniprot.org/core/author | "Lindahl M."xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://purl.uniprot.org/core/author | "Ghafouri B."xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://purl.uniprot.org/core/author | "Ghafouri B."xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://purl.uniprot.org/core/author | "Kihlstroem E."xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://purl.uniprot.org/core/author | "Kihlstroem E."xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://purl.uniprot.org/core/author | "Tagesson C."xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://purl.uniprot.org/core/author | "Tagesson C."xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15158712 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15158712 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://purl.uniprot.org/core/pages | "57-63"xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://purl.uniprot.org/core/pages | "57-63"xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://purl.uniprot.org/core/title | "PLUNC in human nasal lavage fluid: multiple isoforms that bind to lipopolysaccharide."xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://purl.uniprot.org/core/title | "PLUNC in human nasal lavage fluid: multiple isoforms that bind to lipopolysaccharide."xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://purl.uniprot.org/core/volume | "1699"xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://purl.uniprot.org/core/volume | "1699"xsd:string |
| http://purl.uniprot.org/citations/15158712 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15158712 |
| http://purl.uniprot.org/citations/15158712 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15158712 |