| http://purl.uniprot.org/citations/15163637 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15163637 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15163637 | http://www.w3.org/2000/01/rdf-schema#comment | "T-STAR is one of three members of the SAM68 family of RNA-binding proteins that have been shown to be involved in various gene expression pathways including the control of pre-mRNA splicing. We employed a two-hybrid screen to identify proteins that interact with human T-STAR. The predominant interacting proteins were the E3 ubiquitin ligases SIAH1 and SIAH2. We found that SIAH1 bound to an octapeptide sequence in T-STAR targeting it for proteasome-dependent degradation. Rodent T-STAR orthologues (also known as etoile or SLM2) were not targeted for degradation by SIAH1. However a double amino acid substitution of mouse T-STAR that mimics the human SIAH1-binding site brought mouse T-STAR under in vivo control of SIAH1. Using a minigene transfection assay for alternative splicing activity we showed that human T-STAR, like its rodent orthologues can influence splice site choice and that human, but not mouse, T-STAR-dependent alternative splicing is modulated by SIAH1. Western blots of protein from purified germ cells indicated that SIAH1 protein expression peaks in meiosis. In mouse, T-STAR is co-expressed with SIAH1 during meiosis but, in humans, T-STAR is only strongly expressed after meiosis. Comparative sequence analysis showed SIAH-mediated proteasomal degradation of T-STAR has evolved in the primate lineage. Collectively these data suggest that SIAH-mediated down regulation of alternative splicing may be an important developmental difference between otherwise highly conserved T-STAR proteins."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.org/dc/terms/identifier | "doi:10.1093/hmg/ddh165"xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.org/dc/terms/identifier | "doi:10.1093/hmg/ddh165"xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/author | "Saunders P.T."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/author | "Saunders P.T."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/author | "Jones K.T."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/author | "Jones K.T."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/author | "Elliott D.J."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/author | "Elliott D.J."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/author | "Paronetto M.P."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/author | "Paronetto M.P."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/author | "Sette C."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/author | "Sette C."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/author | "Venables J.P."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/author | "Venables J.P."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/author | "Dalgliesh C."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/author | "Dalgliesh C."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/author | "Skitt L."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/author | "Skitt L."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/author | "Thornton J.K."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/author | "Thornton J.K."xsd:string |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15163637 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |