| http://purl.uniprot.org/citations/15170389 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15170389 | http://www.w3.org/2000/01/rdf-schema#comment | "Gab2 (Grb2-associated binder-2), a member of the IRS (insulin receptor substrate)/Gab family of adapter proteins, undergoes tyrosine phosphorylation in response to cytokine or growth factor stimulation and serves as a docking platform for many signal transduction effectors, including the tyrosine phosphatase SHP-2 [SH2 (Src homology 2)-domain-containing tyrosine phosphatase]. Here, we report that, following IL-2 (interleukin-2) stimulation of human T lymphocytes, SHP-2 binds tyrosine residues 614 and 643 of human Gab2 through its N- and C-terminal SH2 domains respectively. However, the sole mutation of Tyr-614 into phenylalanine is sufficient to prevent Gab2 from recruiting SHP-2. Expression of the Gab2 Tyr-614-->Phe (Y614F) mutant, defective in SHP-2 association, prevents ERK (extracellular-signal-regulated kinase) activation and expression of a luciferase reporter plasmid driven by the c-fos SRE (serum response element), indicating that interaction of SHP-2 with Gab2 is required for ERK activation in response to IL-2. Further investigation of IL-2-dependent induction of SRE showed that expression of a constitutively active mutant of the RhoA GTPase synergizes with IL-2 for SRE-driven transcription, whereas a dominant-negative mutant reduces the IL-2 response. Thus, in response to IL-2, full induction of the SRE requires ERK-dependent as well as Rho-dependent signals that target the Ets-box and the CArG-box respectively. We also report that the synergy between Gab2/SHP-2 and RhoA for IL-2-dependent CArG-box-driven transcription depends upon MEK (mitogen-activated protein kinase/ERK kinase) activation, and is likely to involve regulation of the serum response factor co-activator MAL. Our studies thus provide new insights into the role of Gab2 and SHP-2 in IL-2 signal transduction."xsd:string |
| http://purl.uniprot.org/citations/15170389 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj20040103"xsd:string |
| http://purl.uniprot.org/citations/15170389 | http://purl.uniprot.org/core/author | "Bernard O."xsd:string |
| http://purl.uniprot.org/citations/15170389 | http://purl.uniprot.org/core/author | "Courtois G."xsd:string |
| http://purl.uniprot.org/citations/15170389 | http://purl.uniprot.org/core/author | "Arnaud M."xsd:string |
| http://purl.uniprot.org/citations/15170389 | http://purl.uniprot.org/core/author | "Gesbert F."xsd:string |
| http://purl.uniprot.org/citations/15170389 | http://purl.uniprot.org/core/author | "Bertoglio J."xsd:string |
| http://purl.uniprot.org/citations/15170389 | http://purl.uniprot.org/core/author | "Vermot-Desroches C."xsd:string |
| http://purl.uniprot.org/citations/15170389 | http://purl.uniprot.org/core/author | "Wijdenes J."xsd:string |
| http://purl.uniprot.org/citations/15170389 | http://purl.uniprot.org/core/author | "Guenzi C."xsd:string |
| http://purl.uniprot.org/citations/15170389 | http://purl.uniprot.org/core/author | "Mzali R."xsd:string |
| http://purl.uniprot.org/citations/15170389 | http://purl.uniprot.org/core/author | "Crouin C."xsd:string |
| http://purl.uniprot.org/citations/15170389 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15170389 | http://purl.uniprot.org/core/name | "Biochem J"xsd:string |
| http://purl.uniprot.org/citations/15170389 | http://purl.uniprot.org/core/pages | "545-556"xsd:string |
| http://purl.uniprot.org/citations/15170389 | http://purl.uniprot.org/core/title | "Interaction of the tyrosine phosphatase SHP-2 with Gab2 regulates Rho-dependent activation of the c-fos serum response element by interleukin-2."xsd:string |
| http://purl.uniprot.org/citations/15170389 | http://purl.uniprot.org/core/volume | "382"xsd:string |
| http://purl.uniprot.org/citations/15170389 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15170389 |
| http://purl.uniprot.org/citations/15170389 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15170389 |
| http://purl.uniprot.org/uniprot/#_Q06124-mappedCitation-15170389 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15170389 |
| http://purl.uniprot.org/uniprot/#_Q9UQC2-mappedCitation-15170389 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15170389 |
| http://purl.uniprot.org/uniprot/Q06124 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15170389 |
| http://purl.uniprot.org/uniprot/Q9UQC2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15170389 |