| http://purl.uniprot.org/citations/15294977 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15294977 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15294977 | http://www.w3.org/2000/01/rdf-schema#comment | "Granulysin and NK-lysin are antimicrobial proteins found in the granules of human and swine cytotoxic lymphocytes. A murine counterpart to granulysin has not been identified to date, indicating the importance of additional models to fully characterize the role of granulysin-like molecules in the immune response to infectious disease. Two partial nucleotide sequences corresponding to the complete functional domain of granulysin and NK-lysin were amplified from bovine PBMC mRNA. Following stimulation with phorbol ester and calcium ionophore, expression of the bovine gene was detected in CD3(+) T cells, CD4(+) T cells, CD8(+) T cells, WC1(+) gammadelta T cells, and PBMC depleted of CD3(+) T cells, but was absent in CD21(+) cells and CD14(+) cells. Intracellular flow cytometry and immunoblotting confirmed the presence of protein corresponding to the bovine granulysin homologue in activated T lymphocytes and PBMC. Synthetic human, bovine, and swine peptides corresponding to the C terminus of helix 2 through helix 3 region of granulysin displayed potent antimicrobial activity against Escherichia coli, Salmonella enteritidis, Staphylococcus aureus, and Mycobacterium bovis bacillus Calmette-Guérin. Human and bovine peptides corresponding to helix 2 displayed antimycobacterial activity against M. bovis bacillus Calmette-Guérin. Expression of the bovine gene was detected in laser microscopy-dissected lymph node lesions from an M. bovis-infected animal. The identification of a biologically active bovine homologue to granulysin demonstrates the potential of the bovine model in characterizing the role of granulysin in the immune response to a variety of infectious agents."xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.173.4.2607"xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/author | "Waters W.R."xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/author | "Waters W.R."xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/author | "Furrer J.L."xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/author | "Furrer J.L."xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/author | "McIntosh M.A."xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/author | "McIntosh M.A."xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/author | "Lee D.R."xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/author | "Lee D.R."xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/author | "Estes D.M."xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/author | "Estes D.M."xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/author | "Endsley J.J."xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/author | "Endsley J.J."xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/author | "Endsley M.A."xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/author | "Endsley M.A."xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/author | "Maue A.C."xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/author | "Maue A.C."xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/name | "J. Immunol."xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/15294977 | http://purl.uniprot.org/core/pages | "2607-2614"xsd:string |