| http://purl.uniprot.org/citations/1531650 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1531650 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1531650 | http://www.w3.org/2000/01/rdf-schema#comment | "Chinese hamster ovary cells transfected with a 4.0-kilobase macrophage colony-stimulating factor (M-CSF) cDNA express two different M-CSF species; one has an apparent molecular weight of 85,000 and is identified as a homodimer of a 43-kDa subunit, and the other has an indeterminate structure greater than 200 kDa. In this study, we investigated the structure of the high molecular weight M-CSF by immunochemical procedures. The high molecular weight M-CSF was easily purified, since it bound tightly to DEAE-Sephacel and eluted at a characteristically high salt concentration. The high molecular weight M-CSF migrated as a diffuse band of over than 200,000 on nonreducing sodium dodecyl sulfate-polyacrylamide gels. Analysis of the same samples under reducing conditions revealed that the larger species consisted of a heteromer of the 43- and 150-200-kDa M-CSF subunits. Digestion of the 150-200-kDa M-CSF subunit with chondroitinase, which degrades the chondroitin sulfate glycosaminoglycan chain, yielded a 100 kDa band. This species was secreted instead of 150-200-kDa species when the cells were cultured in the presence of beta-D-xyloside, which inhibits the elongation of the chondroitin sulfate glycosaminoglycan chain in proteoglycans, providing additional evidence for the existence of a chondroitin sulfate chain in the 150-200-kDa M-CSF subunit. Removal of O- and N-linked carbohydrate from the 150-200-kDa subunit yielded a polypeptide chain with a larger molecular mass (approximately 45 kDa) than that of the 43-kDa subunit (approximately 25 kDa). Collectively, these results indicate that the 150-200-kDa M-CSF subunit is a proteoglycan with a core protein that may be an alternatively processed form of M-CSF."xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)42841-4"xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)42841-4"xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/author | "Kawashima T."xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/author | "Kawashima T."xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/author | "Yanai N."xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/author | "Yanai N."xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/author | "Suzu S."xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/author | "Suzu S."xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/author | "Takaku F."xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/author | "Takaku F."xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/author | "Nagata N."xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/author | "Nagata N."xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/author | "Ohtsuki T."xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/author | "Ohtsuki T."xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/author | "Motoyoshi K."xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/author | "Motoyoshi K."xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/author | "Takatsu Z."xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/author | "Takatsu Z."xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/1531650 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |