| http://purl.uniprot.org/citations/15383523 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15383523 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15383523 | http://www.w3.org/2000/01/rdf-schema#comment | "FRET experiments utilizing confocal microscopy or flow cytometry assessed homo- and heterotrimeric association of human tumor necrosis factor receptor-associated factors (TRAF) in living cells. Following transfection of HeLa cells with plasmids expressing CFP- or YFP-TRAF fusion proteins, constitutive homotypic association of TRAF2, -3, and -5 was observed, as well as heterotypic association of TRAF1-TRAF2 and TRAF3-TRAF5. A novel heterotypic association between TRAF2 and -3 was detected and confirmed by immunoprecipitation in Ramos B cells that constitutively express both TRAF2 and -3. Experiments employing deletion mutants of TRAF2 and TRAF3 revealed that this heterotypic interaction minimally involved the TRAF-C domain of TRAF3 as well as the TRAF-N domain and zinc fingers 4 and 5 of TRAF2. A novel flow cytometric FRET analysis utilizing a two-step approach to achieve linked FRET from CFP to YFP to HcRed established that TRAF2 and -3 constitutively form homo- and heterotrimers. The functional importance of TRAF2-TRAF3 heterotrimerization was demonstrated by the finding that TRAF3 inhibited spontaneous NF-kappaB, but not AP-1, activation induced by TRAF2. Ligation of CD40 on Ramos B cells by recombinant CD154 caused TRAF2 and TRAF3 to dissociate, whereas overexpression of TRAF3 in Ramos B cells inhibited CD154-induced TRAF2-mediated activation of NF-kappaB. Together, these results reveal a novel association between TRAF2 and TRAF3 that is mediated by unique portions of each protein and that specifically regulates activation of NF-kappaB, but not AP-1."xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m407284200"xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m407284200"xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://purl.uniprot.org/core/author | "Wu X."xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://purl.uniprot.org/core/author | "Wu X."xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://purl.uniprot.org/core/author | "He L."xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://purl.uniprot.org/core/author | "He L."xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://purl.uniprot.org/core/author | "Lipsky P.E."xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://purl.uniprot.org/core/author | "Lipsky P.E."xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://purl.uniprot.org/core/author | "Grammer A.C."xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://purl.uniprot.org/core/author | "Grammer A.C."xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15383523 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15383523 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://purl.uniprot.org/core/pages | "55855-55865"xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://purl.uniprot.org/core/pages | "55855-55865"xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://purl.uniprot.org/core/title | "TRAF3 forms heterotrimers with TRAF2 and modulates its ability to mediate NF-{kappa}B activation."xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://purl.uniprot.org/core/title | "TRAF3 forms heterotrimers with TRAF2 and modulates its ability to mediate NF-{kappa}B activation."xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/15383523 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15383523 |
| http://purl.uniprot.org/citations/15383523 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15383523 |