http://purl.uniprot.org/citations/15525470 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/15525470 | http://www.w3.org/2000/01/rdf-schema#comment | "AimTo find novel proteins that may bind to alpha1A-adrenergic receptor (alpha1A-AR) and investigate their interactions with the other two alpha1-AR subtypes (alpha1B-AR and alpha1D-AR) with an expectation to provide new leads for the function study of the receptors.MethodsYeast two-hybrid assay was performed to screen a human brain cDNA library using the C terminus of alpha1A-AR (alpha1A-AR-CT) as bait. X-Gal assay and o-nitrophenyl-beta-D-galactopyranoside (ONPG) assay were subsequently conducted to further qualitatively or quantitatively confirm the interactions between receptors and the three identified proteins.Results(1) Selection medium screening identified segments of bone morphogenetic protein-1 (BMP-1), active Bcr-related protein (Abr), and filamin-C as binding partners of alpha1A-AR-CT in yeast cells respectively. Besides, protein segments of BMP-1 and Abr could only specifically interact with alpha1A-AR-CT while filamin-C segment interacted with all three alpha1-AR subtypes. (2) In X-Gal assay, the co-transformants of alpha1A-AR-CT and BMP-1 segments turned strong blue at about 30 min while other positive transformants only developed weak blue at about 5-6 h. (3) In ONPG assay, interaction (shown in beta-galactosidase activity) between alpha1A-AR-CT and BMP-1 segments was about 30 times stronger than that of control (P<0.01), while other positive interactions were only about 2-5 times as strong as those of controls (P<0.05).ConclusionIn yeast cells BMP-1, Abr and/or filamin-C could interact with three alpha1-AR subtypes, among which, interaction between BMP-1 and alpha1A-AR was the strongest while other interactions between proteins and receptors were relatively weak."xsd:string |
http://purl.uniprot.org/citations/15525470 | http://purl.uniprot.org/core/author | "Xu Q."xsd:string |
http://purl.uniprot.org/citations/15525470 | http://purl.uniprot.org/core/author | "Zhang T."xsd:string |
http://purl.uniprot.org/citations/15525470 | http://purl.uniprot.org/core/author | "Zhang Y.Y."xsd:string |
http://purl.uniprot.org/citations/15525470 | http://purl.uniprot.org/core/author | "Chen F.R."xsd:string |
http://purl.uniprot.org/citations/15525470 | http://purl.uniprot.org/core/author | "Han Q.D."xsd:string |
http://purl.uniprot.org/citations/15525470 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
http://purl.uniprot.org/citations/15525470 | http://purl.uniprot.org/core/name | "Acta Pharmacol Sin"xsd:string |
http://purl.uniprot.org/citations/15525470 | http://purl.uniprot.org/core/pages | "1471-1478"xsd:string |
http://purl.uniprot.org/citations/15525470 | http://purl.uniprot.org/core/title | "Yeast two-hybrid screening for proteins that interact with alpha1-adrenergic receptors."xsd:string |
http://purl.uniprot.org/citations/15525470 | http://purl.uniprot.org/core/volume | "25"xsd:string |
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