| http://purl.uniprot.org/citations/15537668 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15537668 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15537668 | http://www.w3.org/2000/01/rdf-schema#comment | "Pure hereditary spastic paraplegia is characterized by length-dependent degeneration of the distal ends of long axons. Mutations in spastin are the most common cause of the condition. We set out to investigate the function of spastin using a yeast two-hybrid approach to identify interacting proteins. Using full-length spastin as bait, we identified CHMP1B, a protein associated with the ESCRT (endosomal sorting complex required for transport)-III complex, as a binding partner. Several different approaches confirmed the physiological relevance of the interaction in mammalian cells. Epitope-tagged CHMP1B and spastin showed clear cytoplasmic co-localization in Cos-7 and PC12 cells. CHMP1B and spastin interacted specifically in vitro and in vivo in beta-lactamase protein fragment complementation assays, and spastin co-immunoprecipitated with CHMP1B. The interaction was mediated by a region of spastin lying between residues 80 and 196 and containing a microtubule interacting and trafficking domain. Expression of epitope-tagged CHMP1B in mammalian cells prevented the development of the abnormal microtubule phenotype associated with expression of ATPase-defective spastin. These data point to a role for spastin in intracellular membrane traffic events and provide further evidence to support the emerging recognition that defects in intracellular membrane traffic are a significant cause of motor neuron pathology."xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.org/dc/terms/identifier | "doi:10.1093/hmg/ddi003"xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.org/dc/terms/identifier | "doi:10.1093/hmg/ddi003"xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/author | "Brown S.E."xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/author | "Brown S.E."xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/author | "Reid E."xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/author | "Reid E."xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/author | "Sanderson C.M."xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/author | "Sanderson C.M."xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/author | "Connell J.W."xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/author | "Connell J.W."xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/author | "Duley S."xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/author | "Duley S."xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/author | "Edwards T.L."xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/author | "Edwards T.L."xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/name | "Hum. Mol. Genet."xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/name | "Hum. Mol. Genet."xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/pages | "19-38"xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/pages | "19-38"xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/title | "The hereditary spastic paraplegia protein spastin interacts with the ESCRT-III complex-associated endosomal protein CHMP1B."xsd:string |
| http://purl.uniprot.org/citations/15537668 | http://purl.uniprot.org/core/title | "The hereditary spastic paraplegia protein spastin interacts with the ESCRT-III complex-associated endosomal protein CHMP1B."xsd:string |