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http://purl.uniprot.org/citations/15557096http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15557096http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15557096http://www.w3.org/2000/01/rdf-schema#comment"Plant cells produce different microtubule arrays that are essential for cell division and morphogenesis without equivalent in other eukaryotes. Microtubule-associated proteins influence the behavior of microtubules that is presumed to culminate into transitions from one array to another. We analyzed the microtubule-binding properties of three Arabidopsis (Arabidopsis thaliana) members, AtMAP65-1, AtMAP65-4, and AtMAP65-5, in live cells using laser scanning confocal microscopy. Depending on the overall organization of the cortical array, AtMAP65-1-GFP (green fluorescent protein) and AtMAP65-5-GFP associated with a subset of microtubules. In cells containing both coaligned and oblique microtubules, AtMAP65-1-GFP and AtMAP65-5-GFP tended to be associated with the coaligned microtubules. Cortical microtubules labeled with AtMAP65-1-GFP and AtMAP65-5-GFP appeared as thick bundles and showed more resistance to microtubule-destabilizing drugs. The polymerization rates of AtMAP65-1-GFP and AtMAP65-5-GFP microtubules were similar to those of tubulin-GFP marked microtubules but were different from AtEB1a-GFP, a microtubule plus-end-binding EB1-like protein that stimulated polymerization. By contrast, depolymerization rates of AtMAP65-1-GFP- and AtMAP65-5-GFP-labeled microtubules were reduced. AtMAP65-1-GFP associated with polymerizing microtubules within a bundle, and with fixed microtubule termini, suggesting that AtMAP65-1's function is to bundle and stabilize adjacent microtubules of the cortex. Polymerization within a bundle took place in either direction so that bundling occurred between parallel or antiparallel aligned microtubules. AtMAP65-4-GFP did not label cortical microtubules or the preprophase band, despite continuous expression driven by the 35S promoter, and its subcellular localization was restricted to microtubules that rearranged to form a spindle and the polar sides of the spindle proper. The expression of AtMAP65-4 peaked at mitosis, in agreement with a function related to spindle formation, whereas AtMAP65-1 and AtMAP65-5 were expressed throughout the cell cycle."xsd:string
http://purl.uniprot.org/citations/15557096http://purl.org/dc/terms/identifier"doi:10.1104/pp.104.051623"xsd:string
http://purl.uniprot.org/citations/15557096http://purl.org/dc/terms/identifier"doi:10.1104/pp.104.051623"xsd:string
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/author"Inze D."xsd:string
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/author"Inze D."xsd:string
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/author"Geelen D."xsd:string
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/author"Geelen D."xsd:string
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/author"Van Damme D."xsd:string
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/author"Van Damme D."xsd:string
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/author"Van Poucke K."xsd:string
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/author"Van Poucke K."xsd:string
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/author"Boutant E."xsd:string
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/author"Boutant E."xsd:string
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/author"Ritzenthaler C."xsd:string
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/author"Ritzenthaler C."xsd:string
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/name"Plant Physiol."xsd:string
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/name"Plant Physiol."xsd:string
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/pages"3956-3967"xsd:string
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/pages"3956-3967"xsd:string
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/title"In vivo dynamics and differential microtubule-binding activities of MAP65 proteins."xsd:string
http://purl.uniprot.org/citations/15557096http://purl.uniprot.org/core/title"In vivo dynamics and differential microtubule-binding activities of MAP65 proteins."xsd:string
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