| http://purl.uniprot.org/citations/1560006 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1560006 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1560006 | http://www.w3.org/2000/01/rdf-schema#comment | "Immunoreactive alpha B crystallin and a 28-kDa protein in an extract of human pectoral muscle were precipitated by (NH4)2SO4 at 40% saturation, and coeluted during column chromatography on DEAE-Sepharose and on Bio-Gel A-5m. The two proteins were separated on a column of S-Sepharose HP in the presence of 7 M urea. Further chromatography of each of the two resultant fractions on a column of Superdex 75 pg and on a TSK-SP 5PW column in the presence of urea yielded preparations of alpha B crystallin and the 28-kDa protein each of which gave a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The final preparation of 28-kDa protein contained at least two subtypes, which were separable on the TSK-SP column. However, fragmentation patterns of the two major 28-kDa proteins after digestion with endoproteinase Asp-N were identical. Amino acid sequences of peptides formed by cleavage of the purified 28-kDa protein and alpha B crystallin were identical to those of particular regions of the deduced amino acid sequences of human small heat shock protein (HSP28) and lens alpha B crystallin, respectively. Using an immunoassay method, with antibodies raised in rabbits, we found that HSP28 was present in all human tissues tested and at high levels (greater than 1 micrograms/mg protein) in the heart and other tissues composed of striated and smooth muscles. HSP28, found with alpha B crystallin, in extracts of several human and bovine tissues was trapped on and coeluted with alpha B crystallin from an affinity column prepared with antibodies against alpha B crystallin. This result suggests that the two proteins are associated in cells."xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)42574-4"xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)42574-4"xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/author | "Goto S."xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/author | "Goto S."xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/author | "Kato K."xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/author | "Kato K."xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/author | "Morishita R."xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/author | "Morishita R."xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/author | "Shinohara H."xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/author | "Shinohara H."xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/author | "Asano T."xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/author | "Asano T."xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/author | "Inaguma Y."xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/author | "Inaguma Y."xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/pages | "7718-7725"xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/pages | "7718-7725"xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/title | "Copurification of small heat shock protein with alpha B crystallin from human skeletal muscle."xsd:string |
| http://purl.uniprot.org/citations/1560006 | http://purl.uniprot.org/core/title | "Copurification of small heat shock protein with alpha B crystallin from human skeletal muscle."xsd:string |