| http://purl.uniprot.org/citations/15625105 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15625105 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15625105 | http://www.w3.org/2000/01/rdf-schema#comment | "The spindle midzone, a conspicuous network of antiparallel interdigitating nonkinetochore microtubules between separating chromosomes, plays a crucial role in regulating the initiation and completion of cytokinesis. In this study, we report the use of time-lapse microscopy and a human kinesin endoribonucleases RNase III-prepared short interfering RNA (esiRNA) library to identify Kif4 as a motor protein that translocates PRC1, a spindle midzone-associated cyclin-dependent kinase substrate protein, to the plus ends of interdigitating spindle microtubules during the metaphase-to-anaphase transition. We show that Kif4 binds to PRC1 through its "stalk plus tail" domains and Kif4 and PRC1 colocalize on the spindle midzone/midbody during anaphase and cytokinesis. Suppression of Kif4 expression by Kif4 esiRNA results in the inhibition of PRC1 translocation, a block of the midzone formation, and a failure of cytokinesis. PRC1 translocation and midzone formation can be restored, and the cytokinetic defects can be rescued in Kif4 esiRNA-treated cells by coexpression of Kif4 but not its motor dead mutant Kif4md. Furthermore, we show that cyclin-dependent kinase phosphorylation of PRC1 controls the timing of PRC1 translocation by Kif4. These results, in light of the crucial role of PRC1 in midzone formation, indicate that cell cycle-dependent translocation of PRC1 by Kif4 is essential for midzone formation and cytokinesis."xsd:string |
| http://purl.uniprot.org/citations/15625105 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0408438102"xsd:string |
| http://purl.uniprot.org/citations/15625105 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0408438102"xsd:string |
| http://purl.uniprot.org/citations/15625105 | http://purl.uniprot.org/core/author | "Jiang W."xsd:string |
| http://purl.uniprot.org/citations/15625105 | http://purl.uniprot.org/core/author | "Jiang W."xsd:string |
| http://purl.uniprot.org/citations/15625105 | http://purl.uniprot.org/core/author | "Zhu C."xsd:string |
| http://purl.uniprot.org/citations/15625105 | http://purl.uniprot.org/core/author | "Zhu C."xsd:string |
| http://purl.uniprot.org/citations/15625105 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15625105 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15625105 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/15625105 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/15625105 | http://purl.uniprot.org/core/pages | "343-348"xsd:string |
| http://purl.uniprot.org/citations/15625105 | http://purl.uniprot.org/core/pages | "343-348"xsd:string |
| http://purl.uniprot.org/citations/15625105 | http://purl.uniprot.org/core/title | "Cell cycle-dependent translocation of PRC1 on the spindle by Kif4 is essential for midzone formation and cytokinesis."xsd:string |
| http://purl.uniprot.org/citations/15625105 | http://purl.uniprot.org/core/title | "Cell cycle-dependent translocation of PRC1 on the spindle by Kif4 is essential for midzone formation and cytokinesis."xsd:string |
| http://purl.uniprot.org/citations/15625105 | http://purl.uniprot.org/core/volume | "102"xsd:string |
| http://purl.uniprot.org/citations/15625105 | http://purl.uniprot.org/core/volume | "102"xsd:string |
| http://purl.uniprot.org/citations/15625105 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15625105 |
| http://purl.uniprot.org/citations/15625105 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15625105 |
| http://purl.uniprot.org/citations/15625105 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15625105 |
| http://purl.uniprot.org/citations/15625105 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15625105 |
| http://purl.uniprot.org/uniprot/O95239 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/15625105 |
| http://purl.uniprot.org/uniprot/O43663 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/15625105 |