| http://purl.uniprot.org/citations/1567427 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1567427 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1567427 | http://www.w3.org/2000/01/rdf-schema#comment | "Previously, we reported the importance of Tyr7 for the catalytic activity of human class Pi glutathione S-transferase [Kong et al. (1992) Biochem. Biophys. Res. Comm., 182, 1122]. As an extension of this study, we investigated the pH dependence of kinetic parameters of the wild-type enzyme and the Y7F mutant. The replacement of Tyr7 with phenylalanine was found to alter the pH dependence of Vmax and Vmax/KmCDNB of the enzyme for conjugation of GSH with 1-chloro-2,4-dinitrobenzene (CDNB). The pKa of the thiol of GSH in the wild-type enzyme-GSH complex was estimated to be about 2.4 pK units lower than that in the Y7F-GSH complex. Tyr7 is thus considered to be important for catalytic activity in lowering the pKa of the thiol of GSH in the enzyme-GSH complex."xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://purl.org/dc/terms/identifier | "doi:10.1016/0006-291x(92)91177-r"xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://purl.org/dc/terms/identifier | "doi:10.1016/0006-291x(92)91177-r"xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://purl.uniprot.org/core/author | "Inoue H."xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://purl.uniprot.org/core/author | "Inoue H."xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://purl.uniprot.org/core/author | "Takahashi K."xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://purl.uniprot.org/core/author | "Takahashi K."xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://purl.uniprot.org/core/author | "Kong K.H."xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://purl.uniprot.org/core/author | "Kong K.H."xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://purl.uniprot.org/core/author | "Takasu K."xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://purl.uniprot.org/core/author | "Takasu K."xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1567427 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1567427 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://purl.uniprot.org/core/pages | "194-197"xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://purl.uniprot.org/core/pages | "194-197"xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://purl.uniprot.org/core/title | "Tyrosine-7 in human class Pi glutathione S-transferase is important for lowering the pKa of the thiol group of glutathione in the enzyme-glutathione complex."xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://purl.uniprot.org/core/title | "Tyrosine-7 in human class Pi glutathione S-transferase is important for lowering the pKa of the thiol group of glutathione in the enzyme-glutathione complex."xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://purl.uniprot.org/core/volume | "184"xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://purl.uniprot.org/core/volume | "184"xsd:string |
| http://purl.uniprot.org/citations/1567427 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1567427 |
| http://purl.uniprot.org/citations/1567427 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1567427 |