| http://purl.uniprot.org/citations/15689355 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15689355 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15689355 | http://www.w3.org/2000/01/rdf-schema#comment | "Nr2e3 is an orphan nuclear receptor expressed specifically by retinal photoreceptor cells. Mutations in Nr2e3 result in syndromes characterized by excess blue cones and loss of rods: enhanced S-cone syndrome (ESCS) in humans and rd7 in mice. Using yeast two-hybrid screens with Nr2e3 as bait, the cone-rod homeobox protein Crx was identified as an interacting partner of Nr2e3. Immunoprecipitation assays confirmed this Nr2e3-Crx interaction and identified the DNA-binding domain of each protein as the interaction motif. Immunohistochemistry demonstrated that Crx and Nr2e3 are co-expressed by rod photoreceptors and their precursors. Chromatin immunoprecipitation assays on mouse retina demonstrated that Nr2e3 and Crx co-occupy the promoter/enhancer region of several rod and cone genes in the rod photoreceptor cells. The promoter/enhancer occupancy of Nr2e3 is Crx-dependent, suggesting that Nr2e3 is associated with photoreceptor gene targets by interacting with Crx. Transient transfection assays in HEK293 cells demonstrated that Nr2e3 enhances rhodopsin, but represses S- or M-cone opsin transcription when interacting with Crx. Quantitative real-time RT-PCR analysis on postnatal day 28 (P28) retina of the rd7 mouse, which lacks Nr2e3 protein, revealed an up-regulation of cone genes, but down-regulation of rod genes. Several mutant forms of human Nr2e3 identified from ESCS patients showed defects in interacting with Crx and/or in transcriptional regulatory function. Altogether, our findings suggest that Nr2e3 is a dual-function transcriptional regulator that acts in concert with Crx to promote and maintain the function of rod photoreceptors."xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.org/dc/terms/identifier | "doi:10.1093/hmg/ddi070"xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.org/dc/terms/identifier | "doi:10.1093/hmg/ddi070"xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/author | "Ahmad F."xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/author | "Ahmad F."xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/author | "Chen S."xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/author | "Chen S."xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/author | "Liu J."xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/author | "Liu J."xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/author | "Peng G.H."xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/author | "Peng G.H."xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/author | "Ahmad O."xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/author | "Ahmad O."xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/name | "Hum. Mol. Genet."xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/name | "Hum. Mol. Genet."xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/pages | "747-764"xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/pages | "747-764"xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/title | "The photoreceptor-specific nuclear receptor Nr2e3 interacts with Crx and exerts opposing effects on the transcription of rod versus cone genes."xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/title | "The photoreceptor-specific nuclear receptor Nr2e3 interacts with Crx and exerts opposing effects on the transcription of rod versus cone genes."xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/volume | "14"xsd:string |
| http://purl.uniprot.org/citations/15689355 | http://purl.uniprot.org/core/volume | "14"xsd:string |