http://purl.uniprot.org/citations/15713662 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/15713662 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/15713662 | http://www.w3.org/2000/01/rdf-schema#comment | "The kinase activity of positive transcription elongation factor b (P-TEFb), composed of cyclin-dependent kinase 9 and cyclin T1 or T2, is required for the transition of RNA polymerase II into productive elongation. P-TEFb activity has been shown to be negatively regulated by association with the small nuclear RNA 7SK and the HEXIM1 protein. Here, we characterize HEXIM2, a previously predicted protein with sequence similarity to HEXIM1. HEXIM2 is expressed in HeLa and Jurkat cells, and glycerol gradient analysis and immunoprecipitations indicate that HEXIM2, like HEXIM1, has a regulated association with P-TEFb. As HEXIM1 is knocked down, HEXIM2 functionally compensates for its association with P-TEFb. Electrophoretic mobility shift assays and in vitro kinase assays demonstrate that HEXIM2 forms complexes containing 7SK and P-TEFb and, in conjunction with 7SK, inhibits P-TEFb kinase activity. Our results provide strong evidence that HEXIM2 is a regulator of P-TEFb function. Furthermore, our results support the idea that the utilization of HEXIM1 or HEXIM2 to bind and inhibit P-TEFb can be differentially regulated in vivo."xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m500424200"xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m500424200"xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/author | "Li Q."xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/author | "Li Q."xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/author | "Price D.H."xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/author | "Price D.H."xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/author | "Cooper J.J."xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/author | "Cooper J.J."xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/author | "Byers S.A."xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/author | "Byers S.A."xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/author | "Price J.P."xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/author | "Price J.P."xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/pages | "16360-16367"xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/pages | "16360-16367"xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/title | "HEXIM2, a HEXIM1-related protein, regulates positive transcription elongation factor b through association with 7SK."xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/title | "HEXIM2, a HEXIM1-related protein, regulates positive transcription elongation factor b through association with 7SK."xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/volume | "280"xsd:string |
http://purl.uniprot.org/citations/15713662 | http://purl.uniprot.org/core/volume | "280"xsd:string |