| http://purl.uniprot.org/citations/15731013 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15731013 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15731013 | http://www.w3.org/2000/01/rdf-schema#comment | "Keratins 8 and 18 (K8/18) are major components of the intermediate filaments (IFs) of simple epithelia. We report here the identification of a novel protein termed trichoplein. This protein shows a low degree of sequence similarity to trichohyalin, plectin and myosin heavy chain, and is a K8/18-binding protein. Among interactions between trichoplein and various IF proteins that we tested using two-hybrid methods, trichoplein interacted significantly with K16 and K18, and to some extent with K5, K6a, K8 and K14. In in vitro co-sedimentation assays, trichoplein directly binds to K8/18, but not with vimentin, desmin, actin filaments or microtubules. An antibody raised against trichoplein specifically recognized a polypeptide with a relative molecular mass of 61 kDa in cell lysates. Trichoplein was immunoprecipitated using this antibody in a complex with K8/18 and immunostaining revealed that trichoplein colocalized with K8/18 filaments in HeLa cells. In polarized Caco-2 cells, trichoplein colocalized not only with K8/18 filaments in the apical region but also with desmoplakin, a constituent of desmosomes. In the absorptive cells of the small intestine, trichoplein colocalized with K8/18 filaments at the apical cortical region, and was also concentrated at desmosomes. Taken together, these results suggest that trichoplein is a keratin-binding protein that may be involved in the organization of the apical network of keratin filaments and desmosomes in simple epithelial cells."xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.01667"xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.01667"xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/author | "Hayashi Y."xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/author | "Hayashi Y."xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/author | "Nishizawa M."xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/author | "Nishizawa M."xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/author | "Inagaki M."xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/author | "Inagaki M."xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/author | "Nagata K."xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/author | "Nagata K."xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/author | "Yokoyama T."xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/author | "Yokoyama T."xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/author | "Usukura J."xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/author | "Usukura J."xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/author | "Izawa I."xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/author | "Izawa I."xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/author | "Inoko A."xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/author | "Inoko A."xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |
| http://purl.uniprot.org/citations/15731013 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |