| http://purl.uniprot.org/citations/15760339 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15760339 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15760339 | http://www.w3.org/2000/01/rdf-schema#comment | "ATP-binding cassette (ABC) transporters play a pivotal role in physiology and pathology. We identified and cloned two novel mRNA isoforms (ABCB 5alpha and ABCB 5beta) of the ABC transporter ABCB 5 in human melanoma cells. The deduced ABCB 5alpha protein appears to be an altered splice variant containing only a putative ABC, whereas the ABCB 5beta isoform shares approximately 70% similarity with ABCB1 (MDR1) and has a deduced topological arrangement similar to that of the whole carboxyl terminal half of the ABCB1 gene product, P-glycoprotein, including an intact ABC. Northern blot, real-time PCR, and conventional RT-PCR were used to verify the expression profiles of ABCB 5alpha/beta. We found that the melanomas included among the NCI-60 panel of cell lines preferentially expressed both ABCB 5alpha and ABCB 5beta. However, ABCB 5alpha/beta expression was undetectable in two amelanotic melanomas (M14 and LOX-IMVI). The expression profile of ABCB 5alpha/beta in all of the other melanomas of the panel was confirmed both by RT-PCR and by sequencing. Neither ABCB 5alpha nor ABCB 5beta expression was found in normal tissues such as liver, spleen, thymus, kidney, lung, colon, small intestines or placenta. ABCB 5alpha/beta mRNAs were also expressed in normal melanocytes and in retinal pigment epithelial cells, suggesting that ABCB 5alpha/beta expression is pigment cell-specific and might be involved in melanogenesis. Our findings indicate that expression of ABCB 5alpha/beta might possibly provide two novel molecular markers for differential diagnosis of melanomas and constitute potential molecular targets for therapy of melanomas."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1600-0749.2005.00214.x"xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1600-0749.2005.00214.x"xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Hearing V.J."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Hearing V.J."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Valencia J.C."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Valencia J.C."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Annereau J.-P."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Annereau J.-P."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Chen K.G."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Chen K.G."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Gottesman M.M."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Gottesman M.M."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Hooks J.J."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Hooks J.J."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Liang X.-J."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Liang X.-J."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Nagineni C.N."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Nagineni C.N."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Rouzaud F."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Rouzaud F."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Szakacs G."xsd:string |
| http://purl.uniprot.org/citations/15760339 | http://purl.uniprot.org/core/author | "Szakacs G."xsd:string |