| http://purl.uniprot.org/citations/15944198 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15944198 | http://www.w3.org/2000/01/rdf-schema#comment | "Both homozygous (L166P, M26I, deletion) and heterozygous mutations (D149A, A104T) in the DJ-1 gene have been identified in Parkinson's disease (PD) patients. The biochemical function and subcellular localization of DJ-1 protein have not been clarified. To date the localization of DJ-1 protein has largely been described in studies over-expressing tagged DJ-1 protein in vitro. It is not known whether the subcellular localization of over-expressed DJ-1 protein is identical to that of endogenously expressed DJ-1 protein both in vitro and in vivo. To clarify the subcellular localization and function of DJ-1, we generated three highly specific antibodies to DJ-1 protein and investigated the subcellular localization of endogenous DJ-1 protein in both mouse brain tissues and human neuroblastoma cells. We have found that DJ-1 is widely distributed and is highly expressed in the brain. By cell fractionation and immunogold electron microscopy, we have identified an endogenous pool of DJ-1 in mitochondrial matrix and inter-membrane space. To further investigate whether pathogenic mutations might prevent the distribution of DJ-1 to mitochondria, we generated human neuroblastoma cells stably transfected with wild-type (WT) or mutant (M26I, L166P, A104T, D149A) DJ-1 and performed mitochondrial fractionation and confocal co-localization imaging studies. When compared with WT and other mutants, L166P mutant exhibits largely reduced protein level. However, the pathogenic mutations do not alter the distribution of DJ-1 to mitochondria. Thus, DJ-1 is an integral mitochondrial protein that may have important functions in regulating mitochondrial physiology. Our findings of DJ-1's mitochondrial localization may have important implications for understanding the pathogenesis of PD."xsd:string |
| http://purl.uniprot.org/citations/15944198 | http://purl.org/dc/terms/identifier | "doi:10.1093/hmg/ddi211"xsd:string |
| http://purl.uniprot.org/citations/15944198 | http://purl.uniprot.org/core/author | "Zhang L."xsd:string |
| http://purl.uniprot.org/citations/15944198 | http://purl.uniprot.org/core/author | "Moore D.J."xsd:string |
| http://purl.uniprot.org/citations/15944198 | http://purl.uniprot.org/core/author | "Thomas B."xsd:string |
| http://purl.uniprot.org/citations/15944198 | http://purl.uniprot.org/core/author | "Dawson T.M."xsd:string |
| http://purl.uniprot.org/citations/15944198 | http://purl.uniprot.org/core/author | "Dawson V.L."xsd:string |
| http://purl.uniprot.org/citations/15944198 | http://purl.uniprot.org/core/author | "Yu S.W."xsd:string |
| http://purl.uniprot.org/citations/15944198 | http://purl.uniprot.org/core/author | "Ottersen O.P."xsd:string |
| http://purl.uniprot.org/citations/15944198 | http://purl.uniprot.org/core/author | "Shimoji M."xsd:string |
| http://purl.uniprot.org/citations/15944198 | http://purl.uniprot.org/core/author | "Torp R."xsd:string |
| http://purl.uniprot.org/citations/15944198 | http://purl.uniprot.org/core/author | "Marupudi N.I."xsd:string |
| http://purl.uniprot.org/citations/15944198 | http://purl.uniprot.org/core/author | "Torgner I.A."xsd:string |
| http://purl.uniprot.org/citations/15944198 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15944198 | http://purl.uniprot.org/core/name | "Hum Mol Genet"xsd:string |
| http://purl.uniprot.org/citations/15944198 | http://purl.uniprot.org/core/pages | "2063-2073"xsd:string |
| http://purl.uniprot.org/citations/15944198 | http://purl.uniprot.org/core/title | "Mitochondrial localization of the Parkinson's disease related protein DJ-1: implications for pathogenesis."xsd:string |
| http://purl.uniprot.org/citations/15944198 | http://purl.uniprot.org/core/volume | "14"xsd:string |
| http://purl.uniprot.org/citations/15944198 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15944198 |
| http://purl.uniprot.org/citations/15944198 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15944198 |
| http://purl.uniprot.org/uniprot/Q99LX0#attribution-29A9147C2D0B52442E749EA364AE42AA | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15944198 |
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| http://purl.uniprot.org/uniprot/#_A2A813-mappedCitation-15944198 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15944198 |
| http://purl.uniprot.org/uniprot/#_A2A815-mappedCitation-15944198 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15944198 |