| http://purl.uniprot.org/citations/15961389 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15961389 | http://www.w3.org/2000/01/rdf-schema#comment | "This study tests the hypothesis that G-protein-coupled receptor (GPCR) signaling components involved in the regulation of adenylyl cyclase (AC) localize with caveolin (Cav), a protein marker for caveolae, in both cell-surface and intracellular membrane regions. Using sucrose density fractionation of adult cardiac myocytes, we detected Cav-3 in both buoyant membrane fractions (BF) and heavy/non-buoyant fractions (HF); beta2-adrenergic receptors (AR) in BF; and AC5/6, beta1-AR, M4-muscarinic acetylcholine receptors (mAChR), mu-opioid receptors, and Galpha(s) in both BF and HF. In contrast, M2-mAChR, Galpha(i3), and Galpha(i2) were found only in HF. Immunofluorescence microscopy showed co-localization of Cav-3 with AC5/6, Galpha(s), beta2-AR, and mu-opioid receptors in both sarcolemmal and intracellular membranes, whereas M2-mAChR were detected only intracellularly. Immunofluorescence of adult heart revealed a distribution of Cav-3 identical to that in isolated adult cardiac myocytes. Upon immunoelectron microscopy, Cav-3 co-localized with AC5/6 and Galpha(s) in sarcolemmal and intracellular vesicles, the latter closely allied with T-tubules. Cav-3 immunoprecipitates possessed components that were necessary and sufficient for GPCR agonist-promoted stimulation and inhibition of cAMP formation. The distribution of GPCR, G-proteins, and AC with Cav-3 in both sarcolemmal and intracellular T-tubule-associated regions indicates the existence of multiple Cav-3-localized cellular microdomains for signaling by hormones and drugs in the heart."xsd:string |
| http://purl.uniprot.org/citations/15961389 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m502540200"xsd:string |
| http://purl.uniprot.org/citations/15961389 | http://purl.uniprot.org/core/author | "Niesman I.R."xsd:string |
| http://purl.uniprot.org/citations/15961389 | http://purl.uniprot.org/core/author | "Farquhar M.G."xsd:string |
| http://purl.uniprot.org/citations/15961389 | http://purl.uniprot.org/core/author | "Insel P.A."xsd:string |
| http://purl.uniprot.org/citations/15961389 | http://purl.uniprot.org/core/author | "Lai N.C."xsd:string |
| http://purl.uniprot.org/citations/15961389 | http://purl.uniprot.org/core/author | "Roth D.M."xsd:string |
| http://purl.uniprot.org/citations/15961389 | http://purl.uniprot.org/core/author | "Head B.P."xsd:string |
| http://purl.uniprot.org/citations/15961389 | http://purl.uniprot.org/core/author | "Patel H.H."xsd:string |
| http://purl.uniprot.org/citations/15961389 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15961389 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/15961389 | http://purl.uniprot.org/core/pages | "31036-31044"xsd:string |
| http://purl.uniprot.org/citations/15961389 | http://purl.uniprot.org/core/title | "G-protein-coupled receptor signaling components localize in both sarcolemmal and intracellular caveolin-3-associated microdomains in adult cardiac myocytes."xsd:string |
| http://purl.uniprot.org/citations/15961389 | http://purl.uniprot.org/core/volume | "280"xsd:string |
| http://purl.uniprot.org/citations/15961389 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15961389 |
| http://purl.uniprot.org/citations/15961389 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15961389 |
| http://purl.uniprot.org/uniprot/Q03343#attribution-73007450031EF3CF27325D333B3B82B6 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15961389 |
| http://purl.uniprot.org/uniprot/P10608#attribution-73007450031EF3CF27325D333B3B82B6 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15961389 |
| http://purl.uniprot.org/uniprot/P51638#attribution-73007450031EF3CF27325D333B3B82B6 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15961389 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JWA1-mappedCitation-15961389 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15961389 |
| http://purl.uniprot.org/uniprot/#_A0A0G2K429-mappedCitation-15961389 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15961389 |
| http://purl.uniprot.org/uniprot/#_P63095-mappedCitation-15961389 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15961389 |
| http://purl.uniprot.org/uniprot/#_A0A8I5ZVK2-mappedCitation-15961389 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15961389 |
| http://purl.uniprot.org/uniprot/#_A0A8I5ZZ23-mappedCitation-15961389 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15961389 |