| http://purl.uniprot.org/citations/16020546 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16020546 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16020546 | http://www.w3.org/2000/01/rdf-schema#comment | "Unsaturated fatty acids play an important role in the prevention of human diseases such as diabetes, obesity, cancer, and neurodegeneration. However, their oxidation in vivo by acyl-CoA dehydrogenases (ACADs) that catalyze the first step of each cycle of mitochondrial fatty acid beta-oxidation is not entirely understood. Recently, a novel ACAD (ACAD-9) of unknown function that is highly homologous to human very-long-chain acyl-CoA dehydrogenase was identified by large-scale random sequencing. To characterize its enzymatic role, we have expressed ACAD-9 in Escherichia coli, purified it, and determined its pattern of substrate utilization. The N terminus of the mature form of the enzyme was identified by in vitro mitochondrial import studies of precursor protein. A 37-amino acid leader peptide was cleaved sequentially by two mitochondrial peptidases to yield a predicted molecular mass of 65 kDa for the mature subunit. Submitochondrial fractionation studies found native ACAD-9 to be associated with the mitochondrial membrane. Gel filtration analysis indicated that, like very-long-chain acyl-CoA dehydrogenase, ACAD-9 is a dimer, in contrast to the other known ACADs, which are tetramers. Purified mature ACAD-9 had maximal activity with long-chain unsaturated acyl-CoAs as substrates (C16:1-, C18:1-, C18:2-, C22:6-CoA). These results suggest a previously unrecognized role for ACAD-9 in the mitochondrial beta-oxidation of long-chain unsaturated fatty acids. Because of the substrate specificity and abundance of ACAD-9 in brain, we speculate that it may play a role in the turnover of lipid membrane unsaturated fatty acids that are essential for membrane integrity and structure."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m504460200"xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m504460200"xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/author | "He M."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/author | "He M."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/author | "Corydon T.J."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/author | "Corydon T.J."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/author | "Ensenauer R."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/author | "Ensenauer R."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/author | "Goetzman E.S."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/author | "Goetzman E.S."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/author | "Isaya G."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/author | "Isaya G."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/author | "Mohsen A.W."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/author | "Mohsen A.W."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/author | "Vandahl B.B."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/author | "Vandahl B.B."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/author | "Vockley J."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/author | "Vockley J."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/author | "Willard J.M."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/author | "Willard J.M."xsd:string |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16020546 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |