| http://purl.uniprot.org/citations/16046391 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16046391 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16046391 | http://www.w3.org/2000/01/rdf-schema#comment | "Wrch-1 is a Rho family GTPase that shares strong sequence and functional similarity with Cdc42. Like Cdc42, Wrch-1 can promote anchorage-independent growth transformation. We determined that activated Wrch-1 also promoted anchorage-dependent growth transformation of NIH 3T3 fibroblasts. Wrch-1 contains a distinct carboxyl-terminal extension not found in Cdc42, suggesting potential differences in subcellular location and function. Consistent with this, we found that Wrch-1 associated extensively with plasma membrane and endosomes, rather than with cytosol and perinuclear membranes like Cdc42. Like Cdc42, Wrch-1 terminates in a CAAX tetrapeptide (where C is cysteine, A is aliphatic amino acid, and X is any amino acid) motif (CCFV), suggesting that Wrch-1 may be prenylated similarly to Cdc42. Most surprisingly, unlike Cdc42, Wrch-1 did not incorporate isoprenoid moieties, and Wrch-1 membrane localization was not altered by inhibitors of protein prenylation. Instead, we showed that Wrch-1 is modified by the fatty acid palmitate, and pharmacologic inhibition of protein palmitoylation caused mislocalization of Wrch-1. Most interestingly, mutation of the second cysteine of the CCFV motif (CCFV > CSFV), but not the first, abrogated both Wrch-1 membrane localization and transformation. These results suggest that Wrch-1 membrane association, subcellular localization, and biological activity are mediated by a novel membrane-targeting mechanism distinct from that of Cdc42 and other isoprenylated Rho family GTPases."xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m507362200"xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m507362200"xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/author | "Der C.J."xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/author | "Der C.J."xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/author | "Minden A."xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/author | "Minden A."xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/author | "Cox A.D."xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/author | "Cox A.D."xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/author | "Buss J.E."xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/author | "Buss J.E."xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/author | "Shutes A."xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/author | "Shutes A."xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/author | "Weinbaum C.A."xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/author | "Weinbaum C.A."xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/author | "Berzat A.C."xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/author | "Berzat A.C."xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/author | "Chenette E.J."xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/author | "Chenette E.J."xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/16046391 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |