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| http://purl.uniprot.org/citations/16120608 | http://www.w3.org/2000/01/rdf-schema#comment | "Dendritic calcium/calmodulin-dependent protein kinase II (CaMKII) is dynamically targeted to the synapse. We show that CaMKIIalpha is associated with the CaMKII-binding proteins densin-180, the N-methyl-D-aspartate receptor NR2B subunit, and alpha-actinin in postsynaptic density-enriched rat brain fractions. Residues 819-894 within the C-terminal domain of alpha-actinin-2 constitute the minimal CaMKII-binding domain. Similar amounts of Thr286-autophosphorylated CaMKIIalpha holoenzyme [P-T286]CaMKII bind to alpha-actinin-2 as bind to NR2B (residues 1260-1339) or to densin-180 (residues 1247-1495) in glutathione-agarose cosedimentation assays, even though the CaMKII-binding domains share no amino acid sequence similarity. Like NR2B, alpha-actinin-2 binds to representative splice variants of each CaMKII gene (alpha, beta, gamma, and delta), whereas densin-180 binds selectively to CaMKIIalpha. In addition, C-terminal truncated CaMKIIalpha monomers can interact with NR2B and alpha-actinin-2, but not with densin-180. Soluble alpha-actinin-2 does not compete for [P-T286]CaMKII binding to immobilized densin-180 or NR2B. However, soluble densin-180, but not soluble NR2B, increases CaMKII binding to immobilized alpha-actinin-2 by approximately 10-fold in a PDZ domain-dependent manner. A His6-tagged NR2B fragment associates with GST-densin or GST-actinin but only in the presence of [P-T286]CaMKII. Similarly, His6-tagged densin-180 or alpha-actinin fragments associate with GST-NR2B in a [P-T286]CaMKII-dependent manner. In addition, GST-NR2B and His6-tagged alpha-actinin can bind simultaneously to monomeric CaMKII subunits. In combination, these data support a model in which [P-T286]CaMKIIalpha can simultaneously interact with multiple dendritic spine proteins, possibly stabilizing the synaptic localization of CaMKII and/or nucleating a multiprotein synaptic signaling complex."xsd:string |
| http://purl.uniprot.org/citations/16120608 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m502191200"xsd:string |
| http://purl.uniprot.org/citations/16120608 | http://purl.uniprot.org/core/author | "Colbran R.J."xsd:string |
| http://purl.uniprot.org/citations/16120608 | http://purl.uniprot.org/core/author | "Jiao Y."xsd:string |
| http://purl.uniprot.org/citations/16120608 | http://purl.uniprot.org/core/author | "Bartlett R.K."xsd:string |
| http://purl.uniprot.org/citations/16120608 | http://purl.uniprot.org/core/author | "Robison A.J."xsd:string |
| http://purl.uniprot.org/citations/16120608 | http://purl.uniprot.org/core/author | "Bass M.A."xsd:string |
| http://purl.uniprot.org/citations/16120608 | http://purl.uniprot.org/core/author | "MacMillan L.B."xsd:string |
| http://purl.uniprot.org/citations/16120608 | http://purl.uniprot.org/core/author | "Carmody L.C."xsd:string |
| http://purl.uniprot.org/citations/16120608 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/16120608 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/16120608 | http://purl.uniprot.org/core/pages | "35329-35336"xsd:string |
| http://purl.uniprot.org/citations/16120608 | http://purl.uniprot.org/core/title | "Multivalent interactions of calcium/calmodulin-dependent protein kinase II with the postsynaptic density proteins NR2B, densin-180, and alpha-actinin-2."xsd:string |
| http://purl.uniprot.org/citations/16120608 | http://purl.uniprot.org/core/volume | "280"xsd:string |
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