| http://purl.uniprot.org/citations/16303743 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16303743 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16303743 | http://www.w3.org/2000/01/rdf-schema#comment | "We have developed an in silico method of selection of human full-length cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. Fullness rates were increased to about 80% by combination of the oligo-capping method and ATGpr, software for prediction of translation start point and the coding potential. Then, using 5'-end single-pass sequences, cDNAs having the signal sequence were selected by PSORT ('signal sequence trap'). We also applied 'secretion or membrane protein-related keyword trap' based on the result of BLAST search against the SWISS-PROT database for the cDNAs which could not be selected by PSORT. Using the above procedures, 789 cDNAs were primarily selected and subjected to full-length sequencing, and 334 of these cDNAs were finally selected as novel. Most of the cDNAs (295 cDNAs: 88.3%) were predicted to encode secretion or membrane proteins. In particular, 165(80.5%) of the 205 cDNAs selected by PSORT were predicted to have signal sequences, while 70 (54.2%) of the 129 cDNAs selected by 'keyword trap' preserved the secretion or membrane protein-related keywords. Many important cDNAs were obtained, including transporters, receptors, and ligands, involved in significant cellular functions. Thus, an efficient method of selecting secretion or membrane protein-encoding cDNAs was developed by combining the above four procedures."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.org/dc/terms/identifier | "doi:10.1093/dnares/12.2.117"xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.org/dc/terms/identifier | "doi:10.1093/dnares/12.2.117"xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Aotsuka S."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Aotsuka S."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Hayashi K."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Hayashi K."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Hattori A."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Hattori A."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Ishii S."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Ishii S."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Isogai T."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Isogai T."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Kojima S."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Kojima S."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Kawai Y."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Kawai Y."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Kimura K."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Kimura K."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Nagai K."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Nagai K."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Nishikawa T."xsd:string |
| http://purl.uniprot.org/citations/16303743 | http://purl.uniprot.org/core/author | "Nishikawa T."xsd:string |