| http://purl.uniprot.org/citations/16411896 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16411896 | http://www.w3.org/2000/01/rdf-schema#comment | "The cAMP-dependent pathway up-regulates MITF (microphthalmia-associated transcription factor), important for key melanogenic proteins such as tyrosinase, TRP-1 (tyrosinase-related protein 1) and TRP-2. We asked whether MITF is also a key transcription factor for PKC-beta (protein kinase C-beta), required to phosphorylate otherwise inactive tyrosinase. When paired cultures of human melanocytes were treated with isobutylmethylxanthine, known to increase intracellular cAMP, both protein and mRNA levels of PKC-beta were induced by 24 h. To determine whether MITF modulates PKC-beta expression, paired cultures of human melanocytes were transfected with dn-MITF (dominant-negative MITF) or empty control vector. By immunoblotting, PKC-beta protein was reduced by 63+/-3.7% within 48 h. Co-transfection of an expression vector for MITF-M, the MITF isoform specific for pigment cells, or empty control vector with a full-length PKC-beta promoter-CAT (chloramphenicol acetyltransferase) reporter construct (PKC-beta/CAT) into Cos-7 cells showed >60-fold increase in CAT activity. Melanocytes abundantly also expressed MITF-A, as well as the MITF-B and MITF-H isoforms. However, in contrast with MITF-M, MITF-A failed to transactivate co-expressed PKC-beta/CAT or CAT constructs under the control of a full-length tyrosinase promoter. Together, these results demonstrate that MITF, specifically MITF-M, is a key transcription factor for PKC-beta, linking the PKC- and cAMP-dependent pathways in regulation of melanogenesis."xsd:string |
| http://purl.uniprot.org/citations/16411896 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj20051388"xsd:string |
| http://purl.uniprot.org/citations/16411896 | http://purl.uniprot.org/core/author | "Wu H."xsd:string |
| http://purl.uniprot.org/citations/16411896 | http://purl.uniprot.org/core/author | "Wu C."xsd:string |
| http://purl.uniprot.org/citations/16411896 | http://purl.uniprot.org/core/author | "Park H.Y."xsd:string |
| http://purl.uniprot.org/citations/16411896 | http://purl.uniprot.org/core/author | "Gilchrest B.A."xsd:string |
| http://purl.uniprot.org/citations/16411896 | http://purl.uniprot.org/core/author | "Murphy-Smith M."xsd:string |
| http://purl.uniprot.org/citations/16411896 | http://purl.uniprot.org/core/author | "Stachur C.M."xsd:string |
| http://purl.uniprot.org/citations/16411896 | http://purl.uniprot.org/core/author | "Yonemoto L."xsd:string |
| http://purl.uniprot.org/citations/16411896 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16411896 | http://purl.uniprot.org/core/name | "Biochem J"xsd:string |
| http://purl.uniprot.org/citations/16411896 | http://purl.uniprot.org/core/pages | "571-578"xsd:string |
| http://purl.uniprot.org/citations/16411896 | http://purl.uniprot.org/core/title | "MITF mediates cAMP-induced protein kinase C-beta expression in human melanocytes."xsd:string |
| http://purl.uniprot.org/citations/16411896 | http://purl.uniprot.org/core/volume | "395"xsd:string |
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