| http://purl.uniprot.org/citations/16452140 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16452140 | http://www.w3.org/2000/01/rdf-schema#comment | "We report the results of a genetic screen designed to identify transcriptional coregulators of yeast heat-shock factor (HSF). This sequence-specific activator is required to stimulate both basal and induced transcription; however, the identity of factors that collaborate with HSF in governing noninduced heat-shock gene expression is unknown. In an effort to identify these factors, we isolated spontaneous extragenic suppressors of hsp82-deltaHSE1, an allele of HSP82 that bears a 32-bp deletion of its high-affinity HSF-binding site, yet retains its two low-affinity HSF sites. Nearly 200 suppressors of the null phenotype of hsp82-deltaHSE1 were isolated and characterized, and they sorted into six expression without heat-shock element (EWE) complementation groups. Strikingly, all six groups contain alleles of genes that encode subunits of Mediator. Three of the six subunits, Med7, Med10/Nut2, and Med21/Srb7, map to Mediator's middle domain; two subunits, Med14/Rgr1 and Med16/Sin4, to its tail domain; and one subunit, Med19/Rox3, to its head domain. Mutations in genes encoding these factors enhance not only the basal transcription of hsp82-deltaHSE1, but also that of wild-type heat-shock genes. In contrast to their effect on basal transcription, the more severe ewe mutations strongly reduce activated transcription, drastically diminishing the dynamic range of heat-shock gene expression. Notably, targeted deletion of other Mediator subunits, including the negative regulators Cdk8/Srb10, Med5/Nut1, and Med15/Gal11 fail to derepress hsp82-deltaHSE1. Taken together, our data suggest that the Ewe subunits constitute a distinct functional module within Mediator that modulates both basal and induced heat-shock gene transcription."xsd:string |
| http://purl.uniprot.org/citations/16452140 | http://purl.org/dc/terms/identifier | "doi:10.1534/genetics.105.052738"xsd:string |
| http://purl.uniprot.org/citations/16452140 | http://purl.uniprot.org/core/author | "Singh H."xsd:string |
| http://purl.uniprot.org/citations/16452140 | http://purl.uniprot.org/core/author | "Iqbal J."xsd:string |
| http://purl.uniprot.org/citations/16452140 | http://purl.uniprot.org/core/author | "Davis D.A."xsd:string |
| http://purl.uniprot.org/citations/16452140 | http://purl.uniprot.org/core/author | "Erkine A.M."xsd:string |
| http://purl.uniprot.org/citations/16452140 | http://purl.uniprot.org/core/author | "Gross D.S."xsd:string |
| http://purl.uniprot.org/citations/16452140 | http://purl.uniprot.org/core/author | "Duttweiler H.M."xsd:string |
| http://purl.uniprot.org/citations/16452140 | http://purl.uniprot.org/core/author | "Gross R.R."xsd:string |
| http://purl.uniprot.org/citations/16452140 | http://purl.uniprot.org/core/author | "Kremer S.B."xsd:string |
| http://purl.uniprot.org/citations/16452140 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16452140 | http://purl.uniprot.org/core/name | "Genetics"xsd:string |
| http://purl.uniprot.org/citations/16452140 | http://purl.uniprot.org/core/pages | "2169-2184"xsd:string |
| http://purl.uniprot.org/citations/16452140 | http://purl.uniprot.org/core/title | "A functional module of yeast mediator that governs the dynamic range of heat-shock gene expression."xsd:string |
| http://purl.uniprot.org/citations/16452140 | http://purl.uniprot.org/core/volume | "172"xsd:string |
| http://purl.uniprot.org/citations/16452140 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16452140 |
| http://purl.uniprot.org/citations/16452140 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16452140 |
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| http://purl.uniprot.org/uniprot/P47822#attribution-86E788B8B0EC37E3445E5B378A9E6706 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/16452140 |
| http://purl.uniprot.org/uniprot/P32259#attribution-86E788B8B0EC37E3445E5B378A9E6706 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/16452140 |
| http://purl.uniprot.org/uniprot/P19263#attribution-86E788B8B0EC37E3445E5B378A9E6706 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/16452140 |
| http://purl.uniprot.org/uniprot/Q08278#attribution-86E788B8B0EC37E3445E5B378A9E6706 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/16452140 |
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| http://purl.uniprot.org/uniprot/#_Q06213-mappedCitation-16452140 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/16452140 |