| http://purl.uniprot.org/citations/16648470 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16648470 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16648470 | http://www.w3.org/2000/01/rdf-schema#comment | "Nuclear migration and positioning within cells are critical for many developmental processes and are governed by the cytoskeletal network. Although mechanisms of nuclear-cytoskeletal attachment are unclear, growing evidence links a novel family of nuclear envelope (NE) proteins that share a conserved C-terminal SUN (Sad1/UNC-84 homology) domain. Analysis of Caenorhabditis elegans mutants has implicated UNC-84 in actin-mediated nuclear positioning by regulating NE anchoring of a giant actin-binding protein, ANC-1. Here, we report the identification of SUN1 as a lamin A-binding protein in a yeast two-hybrid screen. We demonstrate that SUN1 is an integral membrane protein located at the inner nuclear membrane. While the N-terminal domain of SUN1 is responsible for detergent-resistant association with the nuclear lamina and lamin A binding, lamin A/C expression is not required for SUN1 NE localization. Furthermore, SUN1 does not interact with type B lamins, suggesting that NE localization is ensured by binding to an additional nuclear component(s), most likely chromatin. Importantly, we find that the luminal C-terminal domain of SUN1 interacts with the mammalian ANC-1 homologs nesprins 1 and 2 via their conserved KASH domain. Our data provide evidence of a physical nuclear-cytoskeletal connection that is likely to be a key mechanism in nuclear-cytoplasmic communication and regulation of nuclear position."xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.26.10.3738-3751.2006"xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.26.10.3738-3751.2006"xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/author | "Trembath R.C."xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/author | "Trembath R.C."xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/author | "Lloyd D.J."xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/author | "Lloyd D.J."xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/author | "Shanahan C.M."xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/author | "Shanahan C.M."xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/author | "Fry A.M."xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/author | "Fry A.M."xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/author | "Shackleton S."xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/author | "Shackleton S."xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/author | "Dent C.L."xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/author | "Dent C.L."xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/author | "Haque F."xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/author | "Haque F."xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/author | "Smallwood D.T."xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/author | "Smallwood D.T."xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/16648470 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |