| http://purl.uniprot.org/citations/16696853 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16696853 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16696853 | http://www.w3.org/2000/01/rdf-schema#comment | "The yeast 2-hybrid system was used to identify protein domains involved in the oligomerization of human guanosine 5'-triphosphate (GTP) Cyclohydrolase I (GCH1) and the interaction of GCH1 with its regulatory partner, GCH1 feedback regulatory protein (GFRP). When interpreted within the structural framework derived from crystallography, our results indicate that the GCH1 N-terminal alpha-helices are not the only domains involved in the formation of dimers from monomers and also suggest an important role for the C-terminal alpha-helix in the assembly of dimers to form decamers. Moreover, a previously unknown role of the extended N-terminal alpha-helix in the interaction of GCH1 and GFRP was revealed. To discover novel GCH1 protein binding partners, we used the yeast 2-hybrid system to screen a human brain library with GCH1 N-terminal amino acids 1-96 as prey. This protruding extension of GCH1 contains two canonical Type-I Src homology-3 (SH3) ligand domains located within amino acids 1-42. Our screen yielded seven unique clones that were subsequently shown to require amino acids 1-42 for binding to GCH1. The interaction of one of these clones, Activator of Heat Shock 90 kDa Protein (Aha1), with GCH1 was validated by glutathione-s-transferase (GST) pull-down assay. Although the physiological relevance of the Aha1-GCH1 interaction requires further study, Aha1 may recruit GCH1 into the endothelial nitric oxide synthase/heat shock protein (eNOS/Hsp90) complex to support changes in endothelial nitric oxide production through the local synthesis of BH4."xsd:string |
| http://purl.uniprot.org/citations/16696853 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1471-4159.2006.03836.x"xsd:string |
| http://purl.uniprot.org/citations/16696853 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1471-4159.2006.03836.x"xsd:string |
| http://purl.uniprot.org/citations/16696853 | http://purl.uniprot.org/core/author | "Kapatos G."xsd:string |
| http://purl.uniprot.org/citations/16696853 | http://purl.uniprot.org/core/author | "Kapatos G."xsd:string |
| http://purl.uniprot.org/citations/16696853 | http://purl.uniprot.org/core/author | "Swick L."xsd:string |
| http://purl.uniprot.org/citations/16696853 | http://purl.uniprot.org/core/author | "Swick L."xsd:string |
| http://purl.uniprot.org/citations/16696853 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16696853 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16696853 | http://purl.uniprot.org/core/name | "J. Neurochem."xsd:string |
| http://purl.uniprot.org/citations/16696853 | http://purl.uniprot.org/core/name | "J. Neurochem."xsd:string |
| http://purl.uniprot.org/citations/16696853 | http://purl.uniprot.org/core/pages | "1447-1455"xsd:string |
| http://purl.uniprot.org/citations/16696853 | http://purl.uniprot.org/core/pages | "1447-1455"xsd:string |
| http://purl.uniprot.org/citations/16696853 | http://purl.uniprot.org/core/title | "A yeast 2-hybrid analysis of human GTP cyclohydrolase I protein interactions."xsd:string |
| http://purl.uniprot.org/citations/16696853 | http://purl.uniprot.org/core/title | "A yeast 2-hybrid analysis of human GTP cyclohydrolase I protein interactions."xsd:string |
| http://purl.uniprot.org/citations/16696853 | http://purl.uniprot.org/core/volume | "97"xsd:string |
| http://purl.uniprot.org/citations/16696853 | http://purl.uniprot.org/core/volume | "97"xsd:string |
| http://purl.uniprot.org/citations/16696853 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16696853 |
| http://purl.uniprot.org/citations/16696853 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16696853 |
| http://purl.uniprot.org/citations/16696853 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16696853 |
| http://purl.uniprot.org/citations/16696853 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16696853 |
| http://purl.uniprot.org/uniprot/P30047 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/16696853 |
| http://purl.uniprot.org/uniprot/P30793 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/16696853 |