| http://purl.uniprot.org/citations/16704978 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16704978 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16704978 | http://www.w3.org/2000/01/rdf-schema#comment | "Transmitter release at synapses between nerve cells is spatially restricted to active zones, where synaptic vesicle docking, priming, and Ca2+-dependent fusion take place in a temporally highly coordinated manner. Munc13s are essential for priming synaptic vesicles to a fusion competent state, and their specific active zone localization contributes to the active zone restriction of transmitter release and the speed of excitation-secretion coupling. However, the molecular mechanism of the active zone recruitment of Munc13s is not known. We show here that the active zone recruitment of Munc13 isoforms Munc13-1 and ubMunc13-2 is regulated by their binding to the Rab3A-interacting molecule RIM1alpha, a key determinant of long term potentiation of synaptic transmission at mossy fiber synapses in the hippocampus. We identify a single point mutation in Munc13-1 and ubMunc13-2 (I121N) that, depending on the type of assay used, strongly perturbs or abolishes RIM1alpha binding in vitro and in cultured fibroblasts, and we demonstrate that RIM1alpha binding-deficient ubMunc13-2(I121) is not efficiently recruited to synapses. Moreover, the levels of Munc13-1 and ubMunc13-2 levels are decreased in RIM1alpha-deficient brain, and Munc13-1 is not properly enriched at active zones of mossy fiber terminals of the mouse hippocampus if RIM1alpha is absent. We conclude that one function of the Munc13/RIM1alpha interaction is the active zone recruitment of Munc13-1 and ubMunc13-2."xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m601421200"xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m601421200"xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/author | "Brose N."xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/author | "Brose N."xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/author | "Reim K."xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/author | "Reim K."xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/author | "Varoqueaux F."xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/author | "Varoqueaux F."xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/author | "Kawabe H."xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/author | "Kawabe H."xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/author | "Andrews-Zwilling Y.S."xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/author | "Andrews-Zwilling Y.S."xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/pages | "19720-19731"xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/pages | "19720-19731"xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/title | "Binding to Rab3A-interacting molecule RIM regulates the presynaptic recruitment of Munc13-1 and ubMunc13-2."xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/title | "Binding to Rab3A-interacting molecule RIM regulates the presynaptic recruitment of Munc13-1 and ubMunc13-2."xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/volume | "281"xsd:string |
| http://purl.uniprot.org/citations/16704978 | http://purl.uniprot.org/core/volume | "281"xsd:string |