| http://purl.uniprot.org/citations/16766533 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16766533 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16766533 | http://www.w3.org/2000/01/rdf-schema#comment | "The two membrane proteins, STIM1 and Orai1, have each been shown to be essential for the activation of store-operated channels (SOC). Yet, how these proteins functionally interact is not known. Here, we reveal that STIM1 and Orai1 expressed together reconstitute functional SOCs. Expressed alone, Orai1 strongly reduces store-operated Ca(2+) entry (SOCE) in human embryonic kidney 293 cells and the Ca(2+) release-activated Ca(2+) current (I(CRAC)) in rat basophilic leukemia cells. However, expressed along with the store-sensing STIM1 protein, Orai1 causes a massive increase in SOCE, enhancing the rate of Ca(2+)entry by up to 103-fold. This entry is entirely store-dependent since the same coexpression causes no measurable store-independent Ca(2+) entry. The entry is completely blocked by the SOC blocker, 2-aminoethoxydiphenylborate. Orai1 and STIM1 coexpression also caused a large gain in CRAC channel function in rat basophilic leukemia cells. The close STIM1 homologue, STIM2, inhibited SOCE when expressed alone but coexpressed with Orai1 caused substantial constitutive (store-independent) Ca(2+) entry. STIM proteins are known to mediate Ca(2+) store-sensing and endoplasmic reticulum-plasma membrane coupling with no intrinsic channel properties. Our results revealing a powerful gain in SOC function dependent on the presence of both Orai1 and STIM1 strongly suggest that Orai1 contributes the PM channel component responsible for Ca(2+) entry. The suppression of SOC function by Orai1 overexpression likely reflects a required stoichiometry between STIM1 and Orai1."xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.c600126200"xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.c600126200"xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/author | "Xu W."xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/author | "Xu W."xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/author | "Gill D.L."xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/author | "Gill D.L."xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/author | "Hewavitharana T."xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/author | "Hewavitharana T."xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/author | "Soboloff J."xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/author | "Soboloff J."xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/author | "Spassova M.A."xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/author | "Spassova M.A."xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/author | "Tang X.D."xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/author | "Tang X.D."xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/pages | "20661-20665"xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/pages | "20661-20665"xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/title | "Orai1 and STIM reconstitute store-operated calcium channel function."xsd:string |
| http://purl.uniprot.org/citations/16766533 | http://purl.uniprot.org/core/title | "Orai1 and STIM reconstitute store-operated calcium channel function."xsd:string |