| http://purl.uniprot.org/citations/16799563 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16799563 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16799563 | http://www.w3.org/2000/01/rdf-schema#comment | "The sterol regulatory element binding protein (SREBP) family of transcription activators are critical regulators of cholesterol and fatty acid homeostasis. We previously demonstrated that human SREBPs bind the CREB-binding protein (CBP)/p300 acetyltransferase KIX domain and recruit activator-recruited co-factor (ARC)/Mediator co-activator complexes through unknown mechanisms. Here we show that SREBPs use the evolutionarily conserved ARC105 (also called MED15) subunit to activate target genes. Structural analysis of the SREBP-binding domain in ARC105 by NMR revealed a three-helix bundle with marked similarity to the CBP/p300 KIX domain. In contrast to SREBPs, the CREB and c-Myb activators do not bind the ARC105 KIX domain, although they interact with the CBP KIX domain, revealing a surprising specificity among structurally related activator-binding domains. The Caenorhabditis elegans SREBP homologue SBP-1 promotes fatty acid homeostasis by regulating the expression of lipogenic enzymes. We found that, like SBP-1, the C. elegans ARC105 homologue MDT-15 is required for fatty acid homeostasis, and show that both SBP-1 and MDT-15 control transcription of genes governing desaturation of stearic acid to oleic acid. Notably, dietary addition of oleic acid significantly rescued various defects of nematodes targeted with RNA interference against sbp-1 and mdt-15, including impaired intestinal fat storage, infertility, decreased size and slow locomotion, suggesting that regulation of oleic acid levels represents a physiologically critical function of SBP-1 and MDT-15. Taken together, our findings demonstrate that ARC105 is a key effector of SREBP-dependent gene regulation and control of lipid homeostasis in metazoans."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.org/dc/terms/identifier | "doi:10.1038/nature04942"xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.org/dc/terms/identifier | "doi:10.1038/nature04942"xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Tjian R."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Tjian R."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Yang F."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Yang F."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Yang S."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Yang S."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Wagner G."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Wagner G."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Iyer L."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Iyer L."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Naeaer A.M."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Naeaer A.M."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Hyberts S.G."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Hyberts S.G."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Satterlee J.S."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Satterlee J.S."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Hart A.C."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Hart A.C."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Walker A.K."xsd:string |
| http://purl.uniprot.org/citations/16799563 | http://purl.uniprot.org/core/author | "Walker A.K."xsd:string |