| http://purl.uniprot.org/citations/16824194 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16824194 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16824194 | http://www.w3.org/2000/01/rdf-schema#comment | "The assembly of the prereplicative complex (pre-RC) at the origin of replication in eukaryotes is a highly regulated and highly conserved process that plays a critical role in preventing multiple rounds of DNA replication per cell division cycle. This study analyzes the molecular dynamics of the assembly of Saccharomyces cerevisiae pre-RC in vitro using ARS1 plasmid DNA and yeast whole cell extracts. In addition, pre-RC assembly was reconstituted in vitro using ARS1 DNA and purified origin-recognition complex (ORC), Cdc6p and Cdt1p-Mcm2-7p. The results reveal sequential recruitment of ORC, Cdc6p, Cdt1p and Mcm2-7p on to ARS1 DNA. When Mcm2-7p is maximally loaded, Cdc6p and Cdt1p are released, suggesting that these two proteins are co-ordinately regulated during pre-RC assembly. In extracts from sid2-21 mutant cells that are deficient in CDT1, ORC and Cdc6p bind to ARS1 but Cdt1p and Mcm2-7p do not. However, Mcm2-7p does bind in the presence of exogenous Cdt1p or Cdt1p-Mcm2-7p complex. Cdt1p-Mcm2-7p complex, which was purified from G1-, early S or G2/M-arrested cells, exhibits structure-specific DNA binding, interacting only with bubble- or Y-shape-DNA, but the biological significance of this observation is not yet known."xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2443.2006.00975.x"xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2443.2006.00975.x"xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/author | "Seki T."xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/author | "Seki T."xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/author | "Kojima A."xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/author | "Kojima A."xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/author | "Kawasaki Y."xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/author | "Kawasaki Y."xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/author | "Kim H.D."xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/author | "Kim H.D."xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/author | "Sugino A."xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/author | "Sugino A."xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/name | "Genes Cells"xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/name | "Genes Cells"xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/pages | "745-756"xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/pages | "745-756"xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/title | "Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro."xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/title | "Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro."xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/16824194 | http://purl.uniprot.org/core/volume | "11"xsd:string |