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http://purl.uniprot.org/citations/16865359http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/16865359http://www.w3.org/2000/01/rdf-schema#comment"

Aims/hypothesis

Adipose tissue inflammation has recently been implicated in the pathogenesis of insulin resistance and is probably linked to high local levels of cytokines. IL1B, a proinflammatory cytokine, may participate in this alteration.

Materials and methods

We evaluated the chronic effect (1-10 days) of IL1B (0.1-20 ng/ml) on insulin signalling in differentiating 3T3-F442A and differentiated 3T3-L1 murine adipocytes and in human adipocytes. We also assessed expression of the gene encoding IL1B in adipose tissue of wild-type and insulin-resistant mice (diet-induced and genetically obese ob/ob mice).

Results

IL1B inhibited insulin-induced phosphorylation of the insulin receptor beta subunit, insulin receptor substrate 1, Akt/protein kinase B and extracellular regulated kinase 1/2 in murine and human adipocytes. Accordingly, IL1B suppressed insulin-induced glucose transport and lipogenesis. Long-term treatment of adipose cells with IL1B decreased cellular lipid content. This could result from enhanced lipolysis and/or decreased expression of genes involved in lipid metabolism (acetyl-CoA carboxylase, fatty acid synthase). Down-regulation of peroxisome proliferating-activated receptor gamma and CCAAT/enhancer-binding protein alpha in response to IL1B may have contributed to the altered phenotype of IL1B-treated adipocytes. Moreover, IL1B altered adipocyte differentiation status in long-term cultures. IL1B also decreased the production of adiponectin, an adipocyte-specific protein that plays a positive role in insulin sensitivity. Expression of the gene encoding IL1B was increased in epididymal adipose tissue of obese insulin-resistant mice.

Conclusions/interpretation

IL1B is upregulated in adipose tissue of obese and insulin-resistant mouse models and may play an important role in the development of insulin resistance in murine and human adipose cells."xsd:string
http://purl.uniprot.org/citations/16865359http://purl.org/dc/terms/identifier"doi:10.1007/s00125-006-0335-z"xsd:string
http://purl.uniprot.org/citations/16865359http://purl.uniprot.org/core/author"Caron M."xsd:string
http://purl.uniprot.org/citations/16865359http://purl.uniprot.org/core/author"Yvan-Charvet L."xsd:string
http://purl.uniprot.org/citations/16865359http://purl.uniprot.org/core/author"Capeau J."xsd:string
http://purl.uniprot.org/citations/16865359http://purl.uniprot.org/core/author"Lagathu C."xsd:string
http://purl.uniprot.org/citations/16865359http://purl.uniprot.org/core/author"Quignard-Boulange A."xsd:string
http://purl.uniprot.org/citations/16865359http://purl.uniprot.org/core/author"Bastard J.P."xsd:string
http://purl.uniprot.org/citations/16865359http://purl.uniprot.org/core/author"Maachi M."xsd:string
http://purl.uniprot.org/citations/16865359http://purl.uniprot.org/core/date"2006"xsd:gYear
http://purl.uniprot.org/citations/16865359http://purl.uniprot.org/core/name"Diabetologia"xsd:string
http://purl.uniprot.org/citations/16865359http://purl.uniprot.org/core/pages"2162-2173"xsd:string
http://purl.uniprot.org/citations/16865359http://purl.uniprot.org/core/title"Long-term treatment with interleukin-1beta induces insulin resistance in murine and human adipocytes."xsd:string
http://purl.uniprot.org/citations/16865359http://purl.uniprot.org/core/volume"49"xsd:string
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