| http://purl.uniprot.org/citations/16898873 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16898873 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16898873 | http://www.w3.org/2000/01/rdf-schema#comment | "The elongation of transcription of HIV RNA at the TAR (transactivation-response element) is highly regulated by positive and negative factors. The cellular negative transcription elongation factor NELF (negative elongation factor) was suggested to be involved in transcriptional regulation of HIV-1 (HIV type 1) by binding to the stem of the viral TAR RNA which is synthesized by cellular RNA polymerase II at the viral long terminal repeat. NELF is a heterotetrameric protein consisting of NELF A, B, C or the splice variant D, and E. In the present study, we determined the solution structure of the RRM (RNA-recognition motif) of the RNA-binding subunit NELF E and studied its interaction with the viral TAR RNA. Our results show that the separately expressed recombinant NELF E RRM has alpha-helical and beta-strand elements adopting a betaalphabetabetaalphabeta fold and is able to bind to TAR RNA. Fluorescence equilibrium titrations with fluorescently labelled double- and single-stranded oligoribonucleotides representing the TAR RNA stem imply that NELF E RRM binds to the single-stranded TAR RNAs with K(d) values in the low-micromolar range."xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj20060421"xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj20060421"xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/author | "Rosch P."xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/author | "Rosch P."xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/author | "Schweimer K."xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/author | "Schweimer K."xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/author | "Wohrl B.M."xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/author | "Wohrl B.M."xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/author | "Neumann L."xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/author | "Neumann L."xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/author | "Rao J.N."xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/author | "Rao J.N."xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/author | "Wenzel S."xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/author | "Wenzel S."xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/pages | "449-456"xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/pages | "449-456"xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/title | "Structural studies on the RNA-recognition motif of NELF E, a cellular negative transcription elongation factor involved in the regulation of HIV transcription."xsd:string |
| http://purl.uniprot.org/citations/16898873 | http://purl.uniprot.org/core/title | "Structural studies on the RNA-recognition motif of NELF E, a cellular negative transcription elongation factor involved in the regulation of HIV transcription."xsd:string |