| http://purl.uniprot.org/citations/16935300 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/16935300 | http://www.w3.org/2000/01/rdf-schema#comment | "Dystrophin Dp71 is the main product of the Duchenne muscular dystrophy gene in the brain; however, its function is unknown. To study the role of Dp71 in neuronal cells, we previously generated by antisense treatment PC12 neuronal cell clones with decreased Dp71 expression (antisense-Dp71 cells). PC12 cells express two different splicing isoforms of Dp71, a cytoplasmic variant called Dp71f and a nuclear isoform called Dp71d. We previously reported that antisense-Dp71 cells display deficient adhesion to substrate and reduced immunostaining of beta1-integrin in the cell area contacting the substrate. In this study, we isolated additional antisense-Dp71 clones to analyze in detail the potential involvement of Dp71f isoform with the beta1-integrin adhesion system of PC12 cells. Immunofluorescence analyses as well as immunoprecipitation assays demonstrated that the PC12 cell beta1-integrin adhesion complex is composed of beta1-integrin, talin, paxillin, alpha-actinin, FAK and actin. In addition, our results showed that Dp71f associates with most of the beta1-integrin complex components (beta1-integrin, FAK, alpha-actinin, talin and actin). In the antisense-Dp71 cells, the deficiency of Dp71 provokes a significant reduction of the beta1-integrin adhesion complex and, consequently, the deficient adhesion of these cells to laminin. In vitro binding experiments confirmed the interaction of Dp71f with FAK and beta1-integrin. Our data indicate that Dp71f is a structural component of the beta1-integrin adhesion complex of PC12 cells that modulates PC12 cell adhesion by conferring proper complex assembly and/or maintenance."xsd:string |
| http://purl.uniprot.org/citations/16935300 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2006.07.075"xsd:string |
| http://purl.uniprot.org/citations/16935300 | http://purl.uniprot.org/core/author | "Ortega A."xsd:string |
| http://purl.uniprot.org/citations/16935300 | http://purl.uniprot.org/core/author | "Centeno F."xsd:string |
| http://purl.uniprot.org/citations/16935300 | http://purl.uniprot.org/core/author | "Garrido E."xsd:string |
| http://purl.uniprot.org/citations/16935300 | http://purl.uniprot.org/core/author | "Mornet D."xsd:string |
| http://purl.uniprot.org/citations/16935300 | http://purl.uniprot.org/core/author | "Cisneros B."xsd:string |
| http://purl.uniprot.org/citations/16935300 | http://purl.uniprot.org/core/author | "Cerecedo D."xsd:string |
| http://purl.uniprot.org/citations/16935300 | http://purl.uniprot.org/core/author | "Garcia-Sierra F."xsd:string |
| http://purl.uniprot.org/citations/16935300 | http://purl.uniprot.org/core/author | "Cerna J."xsd:string |
| http://purl.uniprot.org/citations/16935300 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/16935300 | http://purl.uniprot.org/core/name | "J Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/16935300 | http://purl.uniprot.org/core/pages | "954-965"xsd:string |
| http://purl.uniprot.org/citations/16935300 | http://purl.uniprot.org/core/title | "Dystrophin Dp71f associates with the beta1-integrin adhesion complex to modulate PC12 cell adhesion."xsd:string |
| http://purl.uniprot.org/citations/16935300 | http://purl.uniprot.org/core/volume | "362"xsd:string |
| http://purl.uniprot.org/citations/16935300 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/16935300 |
| http://purl.uniprot.org/citations/16935300 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/16935300 |
| http://purl.uniprot.org/uniprot/O35346#attribution-A1DDB942DBD7A678F8092FCA3BD7E5D0 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/16935300 |
| http://purl.uniprot.org/uniprot/P49134#attribution-63BB1B41D6B3C84A06306DBE63D5825D | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/16935300 |
| http://purl.uniprot.org/uniprot/P49134#attribution-A1DDB942DBD7A678F8092FCA3BD7E5D0 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/16935300 |
| http://purl.uniprot.org/uniprot/Q66H76#attribution-A1DDB942DBD7A678F8092FCA3BD7E5D0 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/16935300 |
| http://purl.uniprot.org/uniprot/A6IJ32#attribution-A1DDB942DBD7A678F8092FCA3BD7E5D0 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/16935300 |
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| http://purl.uniprot.org/uniprot/G3V852#attribution-A1DDB942DBD7A678F8092FCA3BD7E5D0 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/16935300 |