| http://purl.uniprot.org/citations/17027228 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17027228 | http://www.w3.org/2000/01/rdf-schema#comment | "The current view of canonical Wnt signalling is that following Wnt binding to its receptors (Frizzled-Lrp5/6), dishevelled (Dvl) becomes hyperphosphorylated, and the signal is transduced to the APC-GSK3beta-axin-beta-catenin multiprotein complex, which subsequently dissociates. As a result beta-catenin is not phosphorylated, escapes proteosomal degradation and activates its target genes after translocation to the nucleus. Here, we analyzed the importance of the Wnt-3a-induced phosphorylation and shift in electrophoretic migration of Dvl (PS-Dvl) for the activation of beta-catenin. Analysis of Wnt-3a time- and dose-responses in a dopaminergic cell line showed that beta-catenin is activated rapidly (within minutes) and at a low dose of Wnt-3a (1 ng/ml). Surprisingly, PS-Dvl appeared only after 30 min and at greater doses (> or =20 ng/ml) of Wnt-3a. Moreover, we found that a casein kinase 1 inhibitor (D4476) or siRNA for casein kinase 1 delta/epsilon (CK1delta/epsilon) blocked the Wnt-3a-induced PS-Dvl. Interestingly, CK1 inhibition or siRNA for CK1delta/epsilon did not ablate the activation of beta-catenin by Wnt-3a, indicating that there is a PS-Dvl-independent path to activate beta-catenin. The increase in beta-catenin activation by Wnt-3a (PS-Dvl-dependent or -independent) were blocked by Dickkopf1 (Dkk1), suggesting that the effect of Wnt-3a is in both cases mediated by Lrp5/6 receptors. Thus, our results show that Wnt-3a rapidly induce a partial activation of beta-catenin in the absence of PS-Dvl at low doses, while at high doses induce a full activation of beta-catenin in a PS-Dvl-dependent manner."xsd:string |
| http://purl.uniprot.org/citations/17027228 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cellsig.2006.08.011"xsd:string |
| http://purl.uniprot.org/citations/17027228 | http://purl.uniprot.org/core/author | "Bryja V."xsd:string |
| http://purl.uniprot.org/citations/17027228 | http://purl.uniprot.org/core/author | "Schulte G."xsd:string |
| http://purl.uniprot.org/citations/17027228 | http://purl.uniprot.org/core/author | "Arenas E."xsd:string |
| http://purl.uniprot.org/citations/17027228 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17027228 | http://purl.uniprot.org/core/name | "Cell Signal"xsd:string |
| http://purl.uniprot.org/citations/17027228 | http://purl.uniprot.org/core/pages | "610-616"xsd:string |
| http://purl.uniprot.org/citations/17027228 | http://purl.uniprot.org/core/title | "Wnt-3a utilizes a novel low dose and rapid pathway that does not require casein kinase 1-mediated phosphorylation of Dvl to activate beta-catenin."xsd:string |
| http://purl.uniprot.org/citations/17027228 | http://purl.uniprot.org/core/volume | "19"xsd:string |
| http://purl.uniprot.org/citations/17027228 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17027228 |
| http://purl.uniprot.org/citations/17027228 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17027228 |
| http://purl.uniprot.org/uniprot/O54908#attribution-E9F0F8AABF1D51364A6035C1C014928B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17027228 |
| http://purl.uniprot.org/uniprot/Q9DC28#attribution-C3E14E3996982E23799165F073F0EDA8 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17027228 |
| http://purl.uniprot.org/uniprot/Q9JMK2#attribution-C3E14E3996982E23799165F073F0EDA8 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17027228 |
| http://purl.uniprot.org/uniprot/P27467#attribution-E9F0F8AABF1D51364A6035C1C014928B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17027228 |
| http://purl.uniprot.org/uniprot/#_A0A2R8VHL1-mappedCitation-17027228 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17027228 |
| http://purl.uniprot.org/uniprot/#_A0A2R8VI54-mappedCitation-17027228 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17027228 |
| http://purl.uniprot.org/uniprot/#_A0A2R8W6S2-mappedCitation-17027228 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17027228 |
| http://purl.uniprot.org/uniprot/#_A0A668KLW3-mappedCitation-17027228 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17027228 |
| http://purl.uniprot.org/uniprot/#_A9J9D8-mappedCitation-17027228 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17027228 |
| http://purl.uniprot.org/uniprot/#_A8Y2Z5-mappedCitation-17027228 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17027228 |
| http://purl.uniprot.org/uniprot/#_A8Y2Z9-mappedCitation-17027228 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17027228 |
| http://purl.uniprot.org/uniprot/#_F6VU53-mappedCitation-17027228 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17027228 |