| http://purl.uniprot.org/citations/17177976 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17177976 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17177976 | http://www.w3.org/2000/01/rdf-schema#comment | "We have found previously that Txk, a member of the Tec family tyrosine kinases, is involved importantly in T helper 1 (Th1) cytokine production. However, how Txk regulates interferon (IFN)-gamma gene transcription in human T lymphocytes was not fully elucidated. In this study, we identified poly(ADP-ribose) polymerase 1 (PARP1) and elongation factor 1alpha (EF-1alpha) as Txk-associated molecules that bound to the Txk responsive element of the IFN-gamma gene promoter. Txk phosphorylated EF-1alpha and PARP1 formed a complex with them, and bound to the IFN-gamma gene promoter in vitro. In particular, the N terminal region containing the DNA binding domain of PARP1 was important for the trimolecular complex formation involving Txk, EF-1alpha and PARP1. Several mutant Txk which lacked kinase activity were unable to form the trimolecular complex. A PARP1 inhibitor, PJ34, suppressed IFN-gamma but not interleukin (IL)-4 production by normal peripheral blood lymphocytes (PBL). Multi-colour confocal analysis revealed that Txk and EF-1alpha located in the cytoplasm in the resting condition. Upon activation, a complex involving Txk, EF-1alpha and PARP1 was formed and was located in the nucleus. Collectively, Txk in combination with EF-1alpha and PARP1 bound to the IFN-gamma gene promoter, and exerted transcriptional activity on the IFN-gamma gene."xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2249.2006.03249.x"xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2249.2006.03249.x"xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://purl.uniprot.org/core/author | "Maruyama T."xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://purl.uniprot.org/core/author | "Maruyama T."xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://purl.uniprot.org/core/author | "Yoshikawa H."xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://purl.uniprot.org/core/author | "Yoshikawa H."xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://purl.uniprot.org/core/author | "Suzuki N."xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://purl.uniprot.org/core/author | "Suzuki N."xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://purl.uniprot.org/core/author | "Nara K."xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://purl.uniprot.org/core/author | "Nara K."xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17177976 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17177976 | http://purl.uniprot.org/core/name | "Clin. Exp. Immunol."xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://purl.uniprot.org/core/name | "Clin. Exp. Immunol."xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://purl.uniprot.org/core/pages | "164-175"xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://purl.uniprot.org/core/pages | "164-175"xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://purl.uniprot.org/core/title | "Txk, a member of the non-receptor tyrosine kinase of the Tec family, forms a complex with poly(ADP-ribose) polymerase 1 and elongation factor 1alpha and regulates interferon-gamma gene transcription in Th1 cells."xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://purl.uniprot.org/core/title | "Txk, a member of the non-receptor tyrosine kinase of the Tec family, forms a complex with poly(ADP-ribose) polymerase 1 and elongation factor 1alpha and regulates interferon-gamma gene transcription in Th1 cells."xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://purl.uniprot.org/core/volume | "147"xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://purl.uniprot.org/core/volume | "147"xsd:string |
| http://purl.uniprot.org/citations/17177976 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17177976 |
| http://purl.uniprot.org/citations/17177976 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17177976 |