| http://purl.uniprot.org/citations/17190833 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17190833 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17190833 | http://www.w3.org/2000/01/rdf-schema#comment | "Mammalian eIF3 is a 700-kDa multiprotein complex essential for initiation of protein synthesis in eukaryotic cells. It consists of 13 subunits (eIF3a to -m), among which eIF3b serves as a major scaffolding protein. Here we report the solution structure of the N-terminal RNA recognition motif of human eIF3b (eIF3b-RRM) determined by NMR spectroscopy. The structure reveals a noncanonical RRM with a negatively charged surface in the beta-sheet area contradictory with potential RNA binding activity. Instead, eIF3j, which is required for stable 40 S ribosome binding of the eIF3 complex, specifically binds to the rear alpha-helices of the eIF3b-RRM, opposite to its beta-sheet surface. Moreover, we identify that an N-terminal 69-amino acid peptide of eIF3j is sufficient for binding to eIF3b-RRM and that this interaction is essential for eIF3b-RRM recruitment to the 40 S ribosomal subunit. Our results provide the first structure of an important subdomain of a core eIF3 subunit and detailed insights into protein-protein interactions between two eIF3 subunits required for stable eIF3 recruitment to the 40 S subunit."xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m610860200"xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m610860200"xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://purl.uniprot.org/core/author | "ElAntak L."xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://purl.uniprot.org/core/author | "ElAntak L."xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://purl.uniprot.org/core/author | "Locker N."xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://purl.uniprot.org/core/author | "Locker N."xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://purl.uniprot.org/core/author | "Lukavsky P.J."xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://purl.uniprot.org/core/author | "Lukavsky P.J."xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://purl.uniprot.org/core/author | "Tzakos A.G."xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://purl.uniprot.org/core/author | "Tzakos A.G."xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17190833 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17190833 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://purl.uniprot.org/core/pages | "8165-8174"xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://purl.uniprot.org/core/pages | "8165-8174"xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://purl.uniprot.org/core/title | "Structure of eIF3b RNA recognition motif and its interaction with eIF3j: structural insights into the recruitment of eIF3b to the 40 S ribosomal subunit."xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://purl.uniprot.org/core/title | "Structure of eIF3b RNA recognition motif and its interaction with eIF3j: structural insights into the recruitment of eIF3b to the 40 S ribosomal subunit."xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://purl.uniprot.org/core/volume | "282"xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://purl.uniprot.org/core/volume | "282"xsd:string |
| http://purl.uniprot.org/citations/17190833 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17190833 |
| http://purl.uniprot.org/citations/17190833 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17190833 |