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http://purl.uniprot.org/citations/17255263http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/17255263http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/17255263http://www.w3.org/2000/01/rdf-schema#comment"

Purpose

To identify novel biomarkers and therapeutic targets for lung cancers, we screened for genes that were highly transactivated in a large proportion of non-small cell lung cancers (NSCLC) using a cDNA microarray representing 27,648 genes.

Experimental design

A gene encoding insulin-like growth factor-II mRNA-binding protein 1 (IMP-1) was selected as a candidate (> or =3-fold expression than in normal lung tissue in about 70% of NSCLCs). Tumor tissue microarray was applied to examine expression of IMP-1 protein in archival lung cancer samples from 267 patients and investigated its clinicopathologic significance. A role of IMP-1 in cancer cell growth and/or survival was examined by small interfering RNA experiments. Cellular invasive activity of IMP-1 on mammalian cells was examined using Matrigel assays. mRNAs associated with IMP-1 in cancer cells were also isolated by RNA immunoprecipitation followed by cDNA microarray analysis.

Results

Positive immunostaining of IMP-1 was correlated with male (P = 0.0001), tumor size (P = 0.0003), non-adenocarcinoma histology (P < 0.0001), smoking history (P = 0.0005), non-well-differentiated tumor grade (P = 0.0001), and poor prognosis (P = 0.0053). Suppression of IMP-1 expression with small interfering RNA effectively suppressed growth of NSCLC cells. In addition, we identified that exogenous expression of IMP-1 increased the migratory activity of mammalian cells. IMP-1 was able to bind to mRNAs encoding a variety of proteins involved in signal transduction, cell cycle progression, cell adhesion and cytoskeleton, and various types of enzymatic activities.

Conclusions

These results suggest that IMP-1 expression is likely to play important roles in lung cancer development and progression, and that IMP-1 is a prognostic biomarker and a promising therapeutic target for lung cancer."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.org/dc/terms/identifier"doi:10.1158/1078-0432.ccr-06-1297"xsd:string
http://purl.uniprot.org/citations/17255263http://purl.org/dc/terms/identifier"doi:10.1158/1078-0432.ccr-06-1297"xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Ito T."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Ito T."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Kato T."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Kato T."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Kondo S."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Kondo S."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Nakamura Y."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Nakamura Y."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Miyamoto M."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Miyamoto M."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Tsuchiya E."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Tsuchiya E."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Ishikawa N."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Ishikawa N."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Daigo Y."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Daigo Y."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Hayama S."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Hayama S."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Yamabuki T."xsd:string
http://purl.uniprot.org/citations/17255263http://purl.uniprot.org/core/author"Yamabuki T."xsd:string
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