| http://purl.uniprot.org/citations/17404307 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17404307 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17404307 | http://www.w3.org/2000/01/rdf-schema#comment | "Allergenic serine proteases are important in the pathogenesis of asthma. One of these, Pen c 13, is the immunodominant allergen produced by Penicillium citrinum. Many serine proteases induce cytokine expression, but whether Pen c 13 does so in human respiratory epithelial cells is not known. In this study, we investigated whether Pen c 13 caused IL-8 release and activated protease-activated receptors (PARs) in airway epithelial cells. In airway-derived A549 cells and normal human airway epithelial cells, Pen c 13 induced IL-8 release in a dose-dependent manner. Pen c 13 also increased IL-8 release in a time-dependent manner in A549 cells. Pen c 13 cleaved PAR-1 and PAR-2 at their activation sites. Treatment with Pen c 13 induced intracellular Ca(2+) mobilization and desensitized the cells to the action of other proteases and PAR-1 and PAR-2 agonists. Moreover, Pen c 13-mediated IL-8 release was significantly decreased in Ca(2+)-free medium and was abolished by the protease inhibitors, PMSF and 4-(2-aminoethyl) benzenesulfonyl fluoride. Blocking Abs against the cleavage sites of PAR-1 and PAR-2, but not of PAR-4, inhibited Pen c 13-induced IL-8 production, as did inhibition of phospholipase C. Pen c 13 induced IL-8 expression via activation of ERK 1/2, and not of p38 and JNK. In addition, treatment of A549 cells or normal human airway epithelial cells with Pen c 13 increased phosphorylation of ERK 1/2 by a Ca(2+)-dependent pathway. These finding show that Pen c 13 induces IL-8 release in airway epithelial cells and that this is dependent on PAR-1 and PAR-2 activation and intracellular calcium."xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.178.8.5237"xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.178.8.5237"xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/author | "Chow L.P."xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/author | "Chow L.P."xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/author | "Su S.N."xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/author | "Su S.N."xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/author | "Yu C.H."xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/author | "Yu C.H."xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/author | "Perng D.W."xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/author | "Perng D.W."xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/author | "Chiu L.L."xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/author | "Chiu L.L."xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/name | "J. Immunol."xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/name | "J. Immunol."xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/pages | "5237-5244"xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/pages | "5237-5244"xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/title | "Mold allergen, pen C 13, induces IL-8 expression in human airway epithelial cells by activating protease-activated receptor 1 and 2."xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/title | "Mold allergen, pen C 13, induces IL-8 expression in human airway epithelial cells by activating protease-activated receptor 1 and 2."xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/volume | "178"xsd:string |
| http://purl.uniprot.org/citations/17404307 | http://purl.uniprot.org/core/volume | "178"xsd:string |